Macrophages in the tumor microenvironment (TME) can serve as potential targets for therapeutic intervention. The aim of this study was to investigate the molecular mechanism by which M2 macrophage-derived exosomes (M2-Ex) affect lung cancer progression through miRNA transport. The THP-1 cells were differentiated into M0 and M2 macrophages. M2-Ex were isolated and identified by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). Cancer tissues and adjacent tissues of non-small-cell lung cancer (NSCLC) patients were collected. H1299 and A549 cells were co-cultured with M2-Ex. Subcutaneous xenograft mouse model was established. miR-3917 is highly expressed in lung cancer tissues and M2-Ex. Interference of miR-3917 in M2-Ex inhibits H1299 cell proliferation, migration and invasion, while overexpression of miR-3917 had the opposite effect in A549 cells. M2-Ex promote tumor growth by delivering miR-3917 in vivo. miR-3917 could target G protein-coupled receptor kinase 6 (GRK6), and interference of miR-3917 in M2-Ex inhibits H1299 cells proliferation, migration and invasion by up-regulating GRK6 level, while overexpression of miR-3917 had the opposite effect in A549 cells. M2-Ex can transfer miR-3917 into lung cancer cells and promote lung cancer progression, providing theoretical basis for the diagnosis and effective treatment of lung cancer.
基金：
National Natural Science Foundation of China [82060515]; Kunming Medical University Applied Basic Research Joint Special Fund General Program [202001AY070001-025]
第一作者机构：[2]920th Hosp Joint Logist Support Force, Dept Med Management, Kunming 650032, Yunnan, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Song Sinuo,Zhao Yunping,Wang Xiaoxing,et al.M2 macrophages-derived exosomal miR-3917 promotes the progression of lung cancer via targeting GRK6[J].BIOLOGICAL CHEMISTRY.2023,404(1):41-57.doi:10.1515/hsz-2022-0162.
APA：
Song, Sinuo,Zhao, Yunping,Wang, Xiaoxing,Tong, Xinghe,Chen, Xiaobo&Xiong, Qiuxia.(2023).M2 macrophages-derived exosomal miR-3917 promotes the progression of lung cancer via targeting GRK6.BIOLOGICAL CHEMISTRY,404,(1)
MLA：
Song, Sinuo,et al."M2 macrophages-derived exosomal miR-3917 promotes the progression of lung cancer via targeting GRK6".BIOLOGICAL CHEMISTRY 404..1(2023):41-57
