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Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis

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收录情况： ◇ ESCI

作者：
Jia Jie[1,2] ; Zhao Yu[1,2] ; Wang Jian-Hua[3] ; Kuang Yi-Qun[1,2] ;
机构： [1]NHC Key Laboratory of Drug Addiction Medicine, First Affiliated Hospital of Kunming Medical University & Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Kunming Medical University, Kunming, Yunnan 650032, China [2]Scientific Research Laboratory Center, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China [3]Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China
出处：
DOI： 10.1016/j.xpro.2023.102222
ISSN：

摘要：
Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows the dissection of transcriptional changes in immune cells with HIV infection. Here, we isolate PBMCs from HIV-infected individuals. After counting the cell number and verifying cell viability, we perform scRNA-seq for PBMCs on the 10× Genomics protocol and the Illumina NovaSeq 6000 sequencing platform. Furthermore, we analyze the function and cellular trajectories of B cell subsets and B cell receptor (BCR) repertoire after filtering raw sequences data and normalizing gene expression. For complete details on the use and execution of this protocol, please refer to Jia et al. (2022).1.Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

基金：
National Natural Science Foundation of China (32060177 and 82260397), the Yunnan Revitalization Talent Support Program (RLQB20220013), the Fund of Yunnan Provincial Clinical Research Center for Skin Immune Diseases (2019ZF012) from the Department of Science and Technology of Yunnan Province, the Joint Special Fund of Department of Science and Technology of Yunnan Province - Kunming Medical University (202201AY070001-046), the Yunnan Fundamental Research Project (202201AU070202), the Department of Education of Yunnan Province (2019Y0352), and the Open Research Fund of Beijing Key Laboratory for HIV/AIDS Research (BJYAHKF2021001).
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外文
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出版当年[2023]版：
Q4 BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q4 BIOCHEMICAL RESEARCH METHODS

影响因子： 最新[2023版] 最新五年平均 出版当年[2023版] 出版当年五年平均 出版前一年[2022版]

第一作者：
第一作者机构： [1]NHC Key Laboratory of Drug Addiction Medicine, First Affiliated Hospital of Kunming Medical University & Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Kunming Medical University, Kunming, Yunnan 650032, China [2]Scientific Research Laboratory Center, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China
共同第一作者：
通讯作者：
通讯机构： [1]NHC Key Laboratory of Drug Addiction Medicine, First Affiliated Hospital of Kunming Medical University & Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Kunming Medical University, Kunming, Yunnan 650032, China [2]Scientific Research Laboratory Center, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China
推荐引用方式(GB/T 7714)：
Jia Jie,Zhao Yu,Wang Jian-Hua,et al.Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis[J].STAR PROTOCOLS.2023,4(2):doi:10.1016/j.xpro.2023.102222.
APA：
Jia Jie,Zhao Yu,Wang Jian-Hua&Kuang Yi-Qun.(2023).Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis.STAR PROTOCOLS,4,(2)
MLA：
Jia Jie,et al."Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis".STAR PROTOCOLS 4..2(2023)

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