AimsThis study aims to explore the role of exosomes from cancer-associated fibroblasts (CAFs) induced by PDGF-BB in promoting the malignancy of oral squamous cell carcinoma (OSCC) and provide new insight into the mechanism of OSCC progression and its treatment.Main methodsExosomes were extracted from human oral mucosa fibroblasts (hOMFs) and CAFs. Differentially expressed miRNAs of exosomes between hOMFs and CAFs were analysed using high-throughput sequencing and self-programmed R software. Cal-27, a human tongue squamous carcinoma cell line, was treated with exosomes. Differentially expressed miRNAs between clinical cancer tissues and adjacent tissues and between hOMF and CAF exosomes were verified by qRT-PCR. The effect of miR-3529-3p on Cal-27 cells was clarified by overexpressing or knocking down miR-3529-3p in Cal-27 cells. Sample expression and differentially expressed miRNA expression were compared between cancer and paracarcinoma tissues.Key findingsWe found that exosomes from CAFs (CAF-Exos) were internalized by tongue squamous carcinoma cells and promoted their proliferation, migration, invasion, and antiapoptotic effects. MiR-3529-3p was a significant differentially expressed miRNA between CAF-Exos and exosomes from hOMFs (hOMF-Exos). The overexpression of miR-3529-3p promoted proliferation, migration, and invasion and inhibited apoptosis of Cal-27 cells.SignificanceThis study explores the role of PDGF-BB-induced CAFs in promoting malignancy in OSCC. This study will provide new insight into the mechanism of OSCC progression and its treatment.
基金：
Regional Fund Project of the National Natural Science Foundation of China [82060423, 82360523, 82060496]; Yunnan Provincial Department of Science,~Technology-Kunming Medical University applied basic research joint special major project [202001AY07001-007]; Talent Program for Revitalizing Yunnan "Famous Doctor Special" [RLMY20220018]; Scientific and Technological Innovation Team for Precision Pathology Diagnosis of Lung Malignant Tumors at Kunming Medical University [CXTD202210]; Yunnan Provincial Education Department Scientific Research Fund [2023Y0786]
第一作者机构：[1]Kunming Med Univ, Dept Dent Res, Affiliated Stomatol Hosp, Kunming 650106, Yunnan, Peoples R China[2]Kunming Med Univ, Yunnan Key Lab Stomatol, Kunming 650106, Yunnan, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Kunming Med Univ, Dept Dent Res, Affiliated Stomatol Hosp, Kunming 650106, Yunnan, Peoples R China[2]Kunming Med Univ, Yunnan Key Lab Stomatol, Kunming 650106, Yunnan, Peoples R China[8]Qujing Med Coll, Dept Dent Res, Qujing 655011, Yunnan, Peoples R China
推荐引用方式(GB/T 7714)：
You Dingyun,Wang Yanghao,Xu Jianguo,et al.MiR-3529-3p from PDGF-BB-induced cancer-associated fibroblast-derived exosomes promotes the malignancy of oral squamous cell carcinoma[J].DISCOVER ONCOLOGY.2023,14(1):166.doi:10.1007/s12672-023-00753-9.
APA：
You, Dingyun,Wang, Yanghao,Xu, Jianguo,Yang, Rongqiang,Wang, Weizhou...&Bian, Li.(2023).MiR-3529-3p from PDGF-BB-induced cancer-associated fibroblast-derived exosomes promotes the malignancy of oral squamous cell carcinoma.DISCOVER ONCOLOGY,14,(1)
MLA：
You, Dingyun,et al."MiR-3529-3p from PDGF-BB-induced cancer-associated fibroblast-derived exosomes promotes the malignancy of oral squamous cell carcinoma".DISCOVER ONCOLOGY 14..1(2023)
