The invasion of pathogens derived from diet or the environment triggers intestinal inflammation, which is a result of the host intestinal barrier damage and immune dysfunction, such as the decrease of host defense peptides (HDPs) and inhibition of cell proliferation. Herein, the siRNA interference demonstrated that a critical lysozyme, which was mainly induced by dietary supplementations of sodium butyrate and forskolin, improved cell proliferation by inhibiting p38 MAPK and JNK signaling pathways and reinforced the intestinal barrier. Moreover, we established a mouse model of intestinal inflammation with Citrobacter rodentium (C. rodentium). It was found that NaBu and FSK stimulated intestinal epithelial proliferation to repair barrier damage, and performed stronger disease resistance to C. rodentium infection on account of the lysozyme upregulation in colon. Therefore, this study revealed that NaBu and FSK prevented or alleviated the development of foodborne infections by promoting lysozyme expression, which may be mediated by the effect of lysozyme on promoting cell proliferation and improving barrier function.
基金：
Joint Project of Kunming Medical University and Science and Technology Department of Yunnan Province [202101AY070001-119]; National Natural Science Foundation of China [31829004, U22A20514, U23A20232]; National Key R&D Program of China [2022YFD1300404]; Pinduoduo-China Agricultural University Research Fund [PC2023A01001]; The 111 Project [B16044]; The 2115 Talent Development Program of China Agricultural University [1041-00109019]
第一作者机构：[1]Kunming Med Univ, Trauma Ctr, Affiliated Hosp 1, Kunming 650032, Yunnan Province, Peoples R China[2]China Agr Univ, Coll Anim Sci & Technol, Dept Anim Nutr & Feed Sci, State Key Lab Anim Nutr & Feeding, Beijing 100193, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Jinping,Wu Jianmin,Lin Zishen,et al.Dietary sodium butyrate and forskolin promote cell proliferation to resist Citrobacter rodentium infection by lysozyme upregulation[J].JOURNAL OF FUNCTIONAL FOODS.2024,112:doi:10.1016/j.jff.2023.105993.
APA：
Wang, Jinping,Wu, Jianmin,Lin, Zishen,Ma, Ning,Men, Zhaoyue...&Zheng, Hongyu.(2024).Dietary sodium butyrate and forskolin promote cell proliferation to resist Citrobacter rodentium infection by lysozyme upregulation.JOURNAL OF FUNCTIONAL FOODS,112,
MLA：
Wang, Jinping,et al."Dietary sodium butyrate and forskolin promote cell proliferation to resist Citrobacter rodentium infection by lysozyme upregulation".JOURNAL OF FUNCTIONAL FOODS 112.(2024)
农林科学