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Remazolam affects the phenotype and function of astrocytes to improve traumatic brain injury by regulating the Cx43

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机构： [1]Emergency Department, The First Affiliated Hospital of Kunming Medical University, No.295 Xichang Road, Kunming, Yunnan 650032, China [2]Department of Critical Medicine, JiangYin People’s Hospital, No.163 Shoushan Road, Jiangyin, Jiangsu 214400, China

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关键词： TBI Remazolam Cx43 Astrocyte Phenotype and function

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To explore the mechanism by which Remazolam affects the phenotype and function of astrocytes to improve traumatic brain injury (TBI).The oxygen -glucose deprivation/recovery (OGD/R) cell model was constructed to simulate the pathological state of astrocytes in a TBI environment. The viability of astrocytes was measured by CCK-8, and the cytoskeleton changes were observed by Phalloidin- TRITC staining. The expressions of differentiation markers, Cx43 and phosphorylated Cx43 (P-Cx43) of A1/A2 astrocytes were detected by Western blot, and the complement C3 and S100A10 of A1/A2 astrocytes were detected by ELISA. The TBI rat model was established. The water content of brain tissue was measured by dry-wet specific gravity method, the pathological morphology of brain tissue in cortical injury area was observed by HE staining method, ROS was detected by fluorescence quantitative method, Cx43 expression was detected by immunohistochemistry method, and the differentiation markers of A1/A2 astrocytes were detected by immunofluorescence.In the TBI environment, astrocytes showed decreased cell viability, blurred skeleton, and increased expression of Cx43. In TBI rats, the water content of brain tissue increased, the brain tissue in the cortex injury area was seriously damaged, ROS and Cx43 expression were significantly increased, and mainly distributed in A2 astrocytes. Remazolam can reverse the above results after the intervention.Remazolam affects the phenotype and function of astrocytes to improve TBI via regulating Cx43, and plays a role in protecting the neurological function of TBI rats.Copyright © 2024. Published by Elsevier Inc.

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大类 | 3 区医学

小类 | 3 区老年医学

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最新[2023]版：

Q2 GERIATRICS & GERONTOLOGY

影响因子： 3.3 最新[2023版] 3.8 最新五年平均 0 出版当年[2024版] 0 出版当年五年平均 3.3 出版前一年[2023版]

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第一作者机构： [1]Emergency Department, The First Affiliated Hospital of Kunming Medical University, No.295 Xichang Road, Kunming, Yunnan 650032, China

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