机构:[1]Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China昆明医科大学附属第一医院内科科室呼吸内科[2]School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan, China
In this study, network pharmacology combined with biological experimental verification was utilized to screen the targets of isoforskolin (ISOF) and investigate the potential underlying mechanism of ISOF against asthma. Asthma-related targets were screened from the Genecards and DisGeNET databases. SEA and Super-PRED databases were used to obtain the targets of ISOF. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were employed to identify enriched regulatory pathways of key targets in ISOF acting on asthma. Then, a protein-protein interaction (PPI) network was constructed via STRING database and hub genes of ISOF against asthma were further screened using molecular docking. Finally, CCK-8, qPCR, and Western blotting were performed to confirm the targets of ISOF in treating asthma. A total of 96 drug potential therapeutic targets from the relevant databases were screened out. KEGG pathway enrichment analysis predicted that the target genes might be involved in the PI3K-Akt pathway. The core targets of ISOF in treating asthma were identified by the PPI network and molecular docking, including MAPK1, mTOR, and NFKB1. Consistently, in vitro experiments showed that ISOF acting on asthma was involved in inflammatory response by reducing the expression of MAPK1, mTOR, and NFKB1. The present study reveals that MAPK1, mTOR, and NFKB1 might be key targets of ISOF in asthma treatment and the anti-asthma effect might be related to the PI3K-AKT signaling pathway.
基金:
University Cooper- ation Fund of Science and Technology Department of Yunnan [202001AY070001-044]
第一作者机构:[1]Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
通讯作者:
推荐引用方式(GB/T 7714):
Fang Yan,Sun Shibo,Xiao Chuang,et al.Exploring the Mechanism of Isoforskolin against Asthma Based on Network Pharmacology and Experimental Verification[J].CRITICAL REVIEWS IN IMMUNOLOGY.2024,44(6):87-98.doi:10.1615/CritRevImmunol.2024050244.
APA:
Fang, Yan,Sun, Shibo,Xiao, Chuang,Li, Min,Zheng, Yuanyuan...&Luo, Zhuang.(2024).Exploring the Mechanism of Isoforskolin against Asthma Based on Network Pharmacology and Experimental Verification.CRITICAL REVIEWS IN IMMUNOLOGY,44,(6)
MLA:
Fang, Yan,et al."Exploring the Mechanism of Isoforskolin against Asthma Based on Network Pharmacology and Experimental Verification".CRITICAL REVIEWS IN IMMUNOLOGY 44..6(2024):87-98