机构：[1]Department of Pediatrics, the First Afliated Hospital of Kunming Medical University and Yunnan Key Laboratory of Laboratory Medicine, 650032, Kunming, China昆明医科大学附属第一医院儿科[2]Department of Gastroenterology, Children’s Hospital of Kunming Medical University, 650034, Kunming, China[3]Yunnan Center for Disease Control and Prevention, 650100, Kunming, China[4]Kunming Institute of Zoology, Chinese Academy of Sciences, 650000, Kunming, China[5]University of Chinese Academy of Sciences, 100049, Beijing, China
Neonatal cholestasis disease (NCD) is a complex and easily mis-diagnosed condition. We analyzed microbiota community structure in feces and measured short-chain fatty acids, bile acids (BAs) and liver function of 12 healthy, 13 NCD, and 13 treated infants after diagnosis. Based on 16S rRNA gene amplicon sequencing and gas-chromatographic-mass-spectrometric analysis of secondary BAs, we identified microbial genera and metabolites that associate with abnormal bile secretion. Streptococcus gallolyticus and Parabacteroides distasonis, and Lactobacillus gasseri had higher relative abundance in healthy and NCD infants respectively. Compared to NCD patients, healthy infants had higher LCA, CDCA and GCDCA fecal concentrations. The three microbial species and three secondary bile acids were selected as potential non-invasive combined biomarkers to diagnose NCD. We propose that microbiota-metabolite combined biomarkers could be used for diagnosis of NCD, and this may contribute to improved early clinical diagnosis of NCD in the future.
基金：
Key discipline construction project of Yunnan Province During The 12th Five-Year Plan Period [YunDegree [2011]17]; Internal Health Research Institute Project in Yunnan Province [2014NS181]
第一作者机构：[1]Department of Pediatrics, the First Afliated Hospital of Kunming Medical University and Yunnan Key Laboratory of Laboratory Medicine, 650032, Kunming, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Pediatrics, the First Afliated Hospital of Kunming Medical University and Yunnan Key Laboratory of Laboratory Medicine, 650032, Kunming, China
推荐引用方式(GB/T 7714)：
Li Meng,Liu Sixiang,Wang Mingying,et al.Gut Microbiota Dysbiosis Associated with Bile Acid Metabolism in Neonatal Cholestasis Disease[J].SCIENTIFIC REPORTS.2020,10(1):doi:10.1038/s41598-020-64728-4.
APA：
Li, Meng,Liu, Sixiang,Wang, Mingying,Hu, Hongwei,Yin, Jianwen...&Huang, Yongkun.(2020).Gut Microbiota Dysbiosis Associated with Bile Acid Metabolism in Neonatal Cholestasis Disease.SCIENTIFIC REPORTS,10,(1)
MLA：
Li, Meng,et al."Gut Microbiota Dysbiosis Associated with Bile Acid Metabolism in Neonatal Cholestasis Disease".SCIENTIFIC REPORTS 10..1(2020)
