Blockage of TGF-alpha Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells
Background. Xuanwei City in Yunnan province has been one of the towns with highest lung cancer mortality in China. The high content of amorphous silica in the bituminous coal from Xuanwei of Yunnan is mainly present as irregular and spherical silica nanoparticles (SiNPs). It has been reported that silica nanoparticles in bituminous coal correlated with the high incidence of lung cancer in Xuanwei. To explore the role and mechanism of SiNPs in the tumorigenesis of lung cancer in Xuanwei, human mononuclear cells (THP-1) and human bronchial epithelial cells (BEAS-2B) were cocultured in a transwell chamber. Combined with Benzo[a]pyrene-7, 8-dihydrodiol-9, and 10-epoxide (BPDE), SiNPs could significantly promote the proliferation and Epithelial-Mesenchymal Transition (EMT) and inhibit apoptosis of BEAS-2B cells and induce the release of TGF- from THP-1 cells. After neutralizing TGF- with antibody, the proliferation and EMT were decreased and enhanced apoptosis of BEAS-2B cells. Furthermore, the results showed that TGF- in the sera of patients with lung adenocarcinoma in Xuanwei were significantly higher than in patients with benign pulmonary lesions in Xuanwei and those with lung adenocarcinoma in outside of Xuanwei of Yunnan. Taken together, our study found that SiNPs promoted the proliferation and EMT of BEAS-2B cells by inducing the release of TGF- from THP-1 cells.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81460357]; Yunnan provincial Health and Family Planning Commission [2014NS150]; Yunnan Provincial High-Level Health Technical Personnel Training Program [H-201625]
语种:
外文
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PubmedID:
中科院(CAS)分区:
出版当年[2020]版:
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学:研究与实验
最新[2023]版:
无
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出版当年[2019]版:
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版:
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构:[1]Yunnan Key Lab Lab Med, Kunming, Yunnan, Peoples R China[2]Kunming Med Univ, Dept Lab Med, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China
通讯作者:
通讯机构:[1]Yunnan Key Lab Lab Med, Kunming, Yunnan, Peoples R China[2]Kunming Med Univ, Dept Lab Med, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China
推荐引用方式(GB/T 7714):
Li YiXun,Li Huan,Duan Yong,et al.Blockage of TGF-alpha Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells[J].BIOMED RESEARCH INTERNATIONAL.2019,2019:doi:10.1155/2019/8231267.
APA:
Li, YiXun,Li, Huan,Duan, Yong,Cai, XueMei,You, DingYun...&Liu, ZiJie.(2019).Blockage of TGF-alpha Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells.BIOMED RESEARCH INTERNATIONAL,2019,
MLA:
Li, YiXun,et al."Blockage of TGF-alpha Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells".BIOMED RESEARCH INTERNATIONAL 2019.(2019)