机构：[a]Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China外科科室普通外科普通外一科(胃肠与疝外科)昆明医科大学附属第一医院[b]Department of Ultrasound, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China医技科室超声科昆明医科大学附属第一医院
Purpose: The aim of this study was to research the function of lncRNA LUCAT1 in gastric cancer. Methods: Human gastric cancer tissues and paracancer tissues were obtained from 98 patients undergoing surgical resection in our hospital. The human gastric cancer cell lines (HGC27, BGC823, MGC803, SGC7901 and AGS), and normal gastric mucosal cell line GSE1 were used to research the role of lncRNA LUCAT1. ShRNAs specifically targeting lncRNA LUCAT1, miR-134-5p mimic, miR-134-5p inhibitor and their related controls were transfected into cells. Quantitative real-time PCR was used to detect the expression of lncRNA LUCAT1, miR134-5p and YWHAZ. The cell proliferation of SGC7901 cells was determined by CCK8 kit. Colony formation assay was undertaken. Cell apoptosis assay was processed using the Annexin V-FITC / propidium iodide (annxinV/PI) apoptosis detection kit. Migration and invasion were detected by transwell assay. Tumor xenograft model was conducted to calculate the size and weight of the tumors. Luciferase reporter assay was used to confirm the interactions among lncRNA LUCAT1, miR-134-5p and YWHAZ. Results: LncRNA LUCAT1 was confirmed to be highly expressed in gastric cancer. Patients with high LUCAT1 level displayed short overall survival and disease-free survival periods. LUCAT1 knockdown or miR-134-5p overexpression decreased the proliferation, colony formation, migration and invasion of SGC7901 cells. Conclusions: LncRNA LUCAT1 could promote proliferation and invasion of gastric cancer by regulating miR-1345p/YWHAZ axis.
基金：
This study was supported by the grant from Health Commission of
Yunnan Province (D-201657).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区医学
小类|2 区药学3 区医学：研究与实验
最新[2023]版：
大类|2 区医学
小类|1 区药学2 区医学：研究与实验
JCR分区：
出版当年[2019]版：
Q1PHARMACOLOGY & PHARMACYQ1MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ1PHARMACOLOGY & PHARMACY
第一作者机构：[a]Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua District, Kunming 650032, China.[*2]Department of Ultrasound, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua District, Kunming 650032, China.
推荐引用方式(GB/T 7714)：
Junlin Chi,Tonglei Liu,Chengmin Shi,et al.Long non-coding RNA LUCAT1 promotes proliferation and invasion in gastric cancer by regulating miR-134-5p/YWHAZ axis[J].BIOMEDICINE & PHARMACOTHERAPY.2019,118:doi:10.1016/j.biopha.2019.109201.
APA：
Junlin Chi,Tonglei Liu,Chengmin Shi,Huayou Luo,Zhizhong Wu...&Yujian Zeng.(2019).Long non-coding RNA LUCAT1 promotes proliferation and invasion in gastric cancer by regulating miR-134-5p/YWHAZ axis.BIOMEDICINE & PHARMACOTHERAPY,118,
MLA：
Junlin Chi,et al."Long non-coding RNA LUCAT1 promotes proliferation and invasion in gastric cancer by regulating miR-134-5p/YWHAZ axis".BIOMEDICINE & PHARMACOTHERAPY 118.(2019)
医学