机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China[2]University of the Chinese Academy of Sciences, Beijing 101407, China[3]First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China昆明医科大学附属第一医院病理科医技科室[4]Department of Breast Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, China[5]Cancer Epigenetics Laboratory, Department of Clinical Oncology, Sir Y. K. Pao Center for Cancer and Li Ka Shing Institute of Health Sciences, Shenzhen Research Institute, Chinese University of Hong Kong, Hong Kong 518172, China深圳市康宁医院深圳医学信息中心[6]Kunming Institute of Zoology–Chinese University of Hong Kong Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology[7]Institute of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Beijing 100101, China
The Krüppel-like factor 5 (KLF5) transcription factor is
highly expressed in basal type breast cancer and promotes breast
cancer cell proliferation, survival, migration, and tumorigenesis.
KLF5 protein stability is regulated by ubiquitination. In
this study, ubiquitin-specific protease 3 (USP3) was identified as
a new KLF5 deubiquitinase by genome-wide siRNA library
screening.Wedemonstrated that USP3 interacts with KLF5 and
stabilizes KLF5 via deubiquitination. USP3 knockdown inhibits
breast cancer cell proliferation in vitro and tumorigenesis in
vivo, which can be partially rescued by ectopic expression of
KLF5. Furthermore, we observed a positive correlation between
USP3 and KLF5 protein expression levels in human breast cancer
samples. These findings suggest that USP3 is a new KLF5
deubiquitinase and that USP3 may represent a potential therapeutic
target for breast cancer.
基金：
National Key R&D Program of China Grant
2018YFC2000400; National Nature Science Foundation of China Grants
81660437 (to W. C.), 81830087, U1602221, and 31771516 (to C. C.),
81772847 (to R. L.), and 81672639 (to Z. Z.); and Science and Technology
Innovation Team of Yunnan Province Grant 2018HC002.
第一作者机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China[2]University of the Chinese Academy of Sciences, Beijing 101407, China[3]First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China
通讯作者：
推荐引用方式(GB/T 7714)：
Wu Yingying,Qin Junying,Li Fubing,et al.USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5[J].JOURNAL OF BIOLOGICAL CHEMISTRY.2019,294(47):17837-17847.doi:10.1074/jbc.RA119.009102.
APA：
Wu, Yingying,Qin, Junying,Li, Fubing,Yang, Chuanyu,Li, Zhen...&Chen, Ceshi.(2019).USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5.JOURNAL OF BIOLOGICAL CHEMISTRY,294,(47)
MLA：
Wu, Yingying,et al."USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5".JOURNAL OF BIOLOGICAL CHEMISTRY 294..47(2019):17837-17847
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