Purpose: To investigate the impact of different hypoxia reoxygenation (HR) times on autophagy of rat cardiomyocytes (H9C2). Methods: Rat cardiomyocytes were randomly divided into normal control group (group A), hypoxia group (group B), 2 h HR group (group C), 12 h HR group (group D), and 24 h HR group (group E). LC3 II/LC3 I was determined via western blotting, and cell viabilities of cardiomyocytes were measured using methyl thiazolyl tetrazolium (MTT) assay. Results: Cell viabilities in HR model groups were significantly lower than those of group A (P<0.05). LC3 II/LC3 I levels in groups B to D were significantly higher than those of group A (P<0.05), and group D showed the highest LC3 II/LC3 I levels. Cell viabilities in groups B to D were significantly lower than those of group A (P<0.05), with group D showing the lowest cell viabilities (P<0.05). Conclusions: Hypoxia can induce autophagy in rat cardiomyocytes, which can be further activated by reoxygenation; most notable after 12 h. Hypoxia-induced cell injury can be aggravated by reoxygenation. The lowest cell viability was observed at 12 h after reoxygenation; however, cell viability can be recovered after 24 h.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81560072, 81760067]; Yunnan Provincial Science and Technology Department [2013FB128, 2014FB037]; Yunnan Provincial Medical Science Leader Training Project [D-201622]
第一作者机构：[1]Department of Cardiology, the First Affiliated Hospital of Kunming Medical University, Kunming, China
通讯作者：
通讯机构：[*1]Department of Cardiology The First Affiliated Hospital of Kunming Medical University No. 295 Xichang Road Kunming 650032 China
推荐引用方式(GB/T 7714)：
Hu Zhao,Cai Hong-Yan,Luo Yun-Yan,et al.Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes[J].ACTA CIRURGICA BRASILEIRA.2018,33(3):223-230.doi:10.1590/s0102-865020180030000004.
APA：
Hu, Zhao,Cai, Hong-Yan,Luo, Yun-Yan,Xiao, Jian-Ming,Li, Lin&Guo, Tao.(2018).Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes.ACTA CIRURGICA BRASILEIRA,33,(3)
MLA：
Hu, Zhao,et al."Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes".ACTA CIRURGICA BRASILEIRA 33..3(2018):223-230
