机构：[1]Experiment Center of Basic Medical Sciences of Kunming Medical University, Kunming, 650500, China[2]Geriatrics Hospital of Yunnan, Kunming, 650033, China[3]Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, China外科科室神经外科神经外一科（神经外科）昆明医科大学附属第一医院[4]Editorial Department of Journal of Kunming Medical University, Kunming, 650500, China[5]The Electron Microscopy Laboratory, Experiment Center for Medical Science Research of Kunming Medical University, Kunming, 650500, China[6]Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming, 650106, China外科科室骨科昆明医科大学附属第一医院
Accumulating evidence indicates that long noncoding RNA (IncRNA) is implicated in human diseases, including cancers. However, how lncRNA regulates glioblastoma (GBM) progression is poorly understood. Our study revealed a novel IncRNA LINC01446 whose expression was elevated in GBM tissues. Besides, high expression of LINC01446 indicated a poor prognosis in GBM patients. Functionally, LINC01446 knockdown dramatically inhibited GBM cell proliferation, arrested cell-cycle progression and attenuated invasion in vitro. Furthermore, the xenograft mouse model showed that LINC01446 silence led to impaired tumor growth in vivo. Mechanistically, bioinformatics analysis showed that LINC01446 acted as a sponge for miR-489-3p which targeted TPT1. Though inhibiting miR-489-3p availability, LINC01446 promoted TPT1 expression in GBM cells. Rescue experiments demonstrated that restoration of TPT1 could significantly rescued the effects of LINC01446 silence or miR-489-3p overexpression. Taken together, this study demonstrates a novel singling pathway of LINC01446/miR-489-3p/TPT1 cascade that regulates GBM progression. (C) 2018 Elsevier Inc. All rights reserved.
基金：
Young Doctor Program of Yunnan Science and Technology Department-Kunming Medical University Applied Basic Research Joint Special Fund [2017Fe467(-144)]
第一作者机构：[1]Experiment Center of Basic Medical Sciences of Kunming Medical University, Kunming, 650500, China[4]Editorial Department of Journal of Kunming Medical University, Kunming, 650500, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming, 650106, Yunnan Province, China.
推荐引用方式(GB/T 7714)：
Zhang Li,Wang Qin,Wang Fei,et al.LncRNA LINC01446 promotes glioblastoma progression by modulating miR-489-3p/TPT1 axis[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2018,503(3):1484-1490.doi:10.1016/j.bbrc.2018.07.067.
APA：
Zhang, Li,Wang, Qin,Wang, Fei,Zhang, Xiang,Tang, Ying&Wang, Shaoyun.(2018).LncRNA LINC01446 promotes glioblastoma progression by modulating miR-489-3p/TPT1 axis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,503,(3)
MLA：
Zhang, Li,et al."LncRNA LINC01446 promotes glioblastoma progression by modulating miR-489-3p/TPT1 axis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 503..3(2018):1484-1490
