Inflammatory bowel disease (IBD) is the result of a chronic intestinal inflammatory response which usually occurred in colon and small intestine. Keratins constitute the intermediate filament cytoskeleton in all epithelia. The present study was intended to explore the role of Keratin 1 (KRT1) in the progress of IBD. In normal intestinal tissue, the expression of KRT1 was detected by RT-PCR and Western blot. The levels of KRT1 protein significantly decreased in serum samples of IBD patients as compared with sera of healthy controls. Immunohistochemistry revealed that the expression of KRT1 decreased in various intestinal diseases, especially in Crohn's disease and ulcerative colitis. Furthermore, down-regulated KRT1 was correlated with the severity of IBD. The overexpression of KRT1 maintained epithelial barrier in Caco-2 cells after IL-beta treatment. Furthermore, IL-beta-induced disruption of tight junction became significantly attenuated in KRT1 over-expressing Caco-2 cells as compared with control cells. Thus, KRT1 played an important role of maintaining epithelial barrier and its down-regulation in intestinal tissue was correlated with the progression of IBD. (C) 2017 Elsevier B.V. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81660100, 81970501, 81260074, 81160055]; Applied Basic Research Key Project of Yunnan Province [2016FA033]; Scientific Research Fund General Projects of Yunnan Provincial Department of Education [2015Y150]; Social Development of Science and Technology Projects of Yunnan Province [2013CA021]; Foundation of Yunnan Institute of Digestive Disease [2014NS123, 2016NS002]; Kunming Engineering Research Center of Digestive Disease [2015-3-A-02243]
第一作者机构：[1]Kunming Med Univ, Yunnan Inst Digest Dis, Dept Gastroenterol, Affiliated Hosp 1, Kunming 650032, Peoples R China
通讯作者：
通讯机构：[1]Kunming Med Univ, Yunnan Inst Digest Dis, Dept Gastroenterol, Affiliated Hosp 1, Kunming 650032, Peoples R China
推荐引用方式(GB/T 7714)：
Dong Xiangqian,Liu Zichao,Lan Danfeng,et al.Critical role of Keratin 1 in maintaining epithelial barrier and correlation of its down-regulation with the progression of inflammatory bowel disease[J].GENE.2017,608:13-19.doi:10.1016/j.gene.2017.01.015.
APA：
Dong, Xiangqian,Liu, Zichao,Lan, Danfeng,Niu, Junkun,Miao, Jiarong...&Miao, Yinglei.(2017).Critical role of Keratin 1 in maintaining epithelial barrier and correlation of its down-regulation with the progression of inflammatory bowel disease.GENE,608,
MLA：
Dong, Xiangqian,et al."Critical role of Keratin 1 in maintaining epithelial barrier and correlation of its down-regulation with the progression of inflammatory bowel disease".GENE 608.(2017):13-19
