机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences &Yunnan Province, Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy ofSciences, Kunming, China[2]Department of Neurosurgery, First People’s Hospital of Honghe State, Honghe, China[3]Department of Neurosurgery, Second Affiliated Hospital of Nanchang University, Nanchang, China[4]Departmentof Neurosurgery, Center People’s Hospital of Zhumadian State, Zhumadian, China[5]Second Department ofNeurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China外科科室神经外科神经外一科（神经外科）昆明医科大学附属第一医院[6]Yunnan Key Laboratoryof Primate Biomedical Research Institute of Primate Translational Medicine, Kunming University of Science andTechnology, Kunming, China[7]State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, ChineseAcademy of Sciences, Beijing, China
Endothelin-1 (ET-1), a vasoconstrictor, has recently been used to induce focal ischemia in rodents and marmoset monkeys. The rhesus monkey, however, has numerous advantages to the rodent and marmoset that make it a superior and irreplaceable animal model for studying stroke in the brain. In the present study, after mapping the preferred hand representation in two healthy male monkeys with intracortical micro-stimulation, ET-1 was microinjected into the contralateral motor cortex (M1) to its preferred hand. The monkeys had been trained in three manual dexterity tasks before the microinjection and were tested for these tasks following the ET-1 injection. Brain Magnetic Resonance Imaging scans were performed 1, 7, 14 and 28 days post ischemia. It was found that ET-1 impaired the manual dexterity of the monkeys in the vertical slot and rotating Brinkman board tasks 3-8 days after the injection. Brain imaging found that severe edema was present 7 days after the focal ischemia. This data suggest that ET-1 can induce transient ischemic stroke in rhesus monkey and that ET-1 induced focal ischemia in non-human primates is a potential model to study the mechanism of stroke and brain repair after stroke.
基金：
National Science Foundation of ChinaNational Natural Science Foundation of China [NSFC 31571109, 31271168]; 973 programNational Basic Research Program of China [2015CB856401]; Yunnan Key Program of Science, Technology [2014FC005]; Chinese-Finnish Joint Project [813111172]; Hainan special fund project for science and technology [KJHZ2015-20]
第一作者机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences &Yunnan Province, Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy ofSciences, Kunming, China[2]Department of Neurosurgery, First People’s Hospital of Honghe State, Honghe, China
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推荐引用方式(GB/T 7714)：
PeiMin Dai,Hui Huang,Lin Zhang,et al.A pilot study on transient ischemic stroke induced with endothelin-1 in the rhesus monkeys[J].SCIENTIFIC REPORTS.2017,7:doi:10.1038/srep45097.
APA：
PeiMin Dai,Hui Huang,Lin Zhang,Jing He,XuDong Zhao...&JianHong Wang.(2017).A pilot study on transient ischemic stroke induced with endothelin-1 in the rhesus monkeys.SCIENTIFIC REPORTS,7,
MLA：
PeiMin Dai,et al."A pilot study on transient ischemic stroke induced with endothelin-1 in the rhesus monkeys".SCIENTIFIC REPORTS 7.(2017)
