机构：[1]Department of Postgraduate, Kunming Medical University, Kunming, Yunnan, China[2]Department of Clinical Laboratory,the First Affliated Hospital of Kunming Medical University,Kunming,Yunnan,China昆明医科大学附属第一医院医技科室医学检验科[3]Department of Pathology, the First People's Hospital of Yunnan Province, Kunming, Yunnan, China云南省第一人民医院[4]Department of Clinical Laboratory, the Affliated Kunhua Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China云南省第一人民医院[5]Department of Neurology, the First People's Hospital of Yunnan Province, Kunming, Yunnan, China云南省第一人民医院[6]Department of Clinical Psychology,the First People's Hospital of Yunnan Province,Kunming,Yunnan,China.云南省第一人民医院[7]Department of Neurology,the Jiujiang No.1 People’s Hospital,Jiujiang,Hubei,China.
Purpose: The effects of cathepsin L on claudin-1 expression were investigated under hyperthermic condition in a blood-brain barrier (BBB) model in vitro, in order to estimate the potential effects of hyperthermia on BBB dysfunction. Materials and methods: Brain microvascular endothelial cells (BMECs) and astrocytes were obtained from rat brain. The BBB models were randomly divided into a sham (37 degrees C) group, a 39 degrees C group, a 37 degrees C+cathepsin L group and a 39 degrees C+cathepsin L group. The permeability of BBB was judged. The expressions of cathepsin L in astrocytes and claudin-1 in BMECs were detected using immunohistochemistry method and western blot assay. Results: The permeability of BBB models was higher in the 39 degrees C group than in the sham group. The cathepsin L expression in astrocytes was higher in the 39 degrees C group than in the sham group (P <0.01), whereas the claudin-1 expression in BMECs was lower in the 39 degrees C group than in the sham group (P<0.01). The claudin-1 expression in BMECs was significantly lower in the 37 degrees C+cathepsin L group than in the sham group (P<0.01). At the same time point, the claudin-1 expression in BMECs was significantly lower in the 39 degrees C+cathepsin L group than in the 37 C+cathepsin L group (P <0.01). Conclusion: Hyperthermia can probably decrease claudin-1 expression in BMECs by upregulating cathepsin L expression in astrocytes in a BBB model in vitro. (C) 2015 Published by Elsevier B.V.
基金：
Applied Basic Research Project in Yunnan Province of China [2011FB148]; Young and Middle-Aged Academic and Technological Leaders and Reserved Talents of Yunnan Province of China [2012HB028]; Health System Leaders of Yunnan Province of China [D-201235]; Academician Wang Long-de Workstation
第一作者机构：[1]Department of Postgraduate, Kunming Medical University, Kunming, Yunnan, China[6]Department of Clinical Psychology,the First People's Hospital of Yunnan Province,Kunming,Yunnan,China.
通讯作者：
推荐引用方式(GB/T 7714)：
Ji Conghua,Wang Limin,Dai Rongrong,et al.Hyperthermia exacerbates the effects of cathepsin L on claudin-1 in a blood-brain barrier model in vitro[J].BRAIN RESEARCH.2016,1631:72-79.doi:10.1016/j.brainres.2015.11.043.
APA：
Ji, Conghua,Wang, Limin,Dai, Rongrong,Shan, Li,Yang, Hui...&Meng, Qiang.(2016).Hyperthermia exacerbates the effects of cathepsin L on claudin-1 in a blood-brain barrier model in vitro.BRAIN RESEARCH,1631,
MLA：
Ji, Conghua,et al."Hyperthermia exacerbates the effects of cathepsin L on claudin-1 in a blood-brain barrier model in vitro".BRAIN RESEARCH 1631.(2016):72-79
