机构:[1]Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China[2]Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou, China[3]Department of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, China内科科室外科科室神经内科泌尿外科昆明医科大学附属第一医院
Neuroadaptations and alterations in neuronal excitability are critical in brain maturation and many neurological diseases. Fragile X syndrome (FXS) is a pervasive neurodevelopmental disorder characterized by extensive synaptic and circuit dysfunction. It is still unclear about the alterations in intrinsic excitability of individual neurons and their link to hyperexcitable circuitry. In this study, whole cell patch-clamp recordings were employed to characterize the membrane and firing properties of layer IV cells in slices of the somatosensory cortex of Fmr-1 knockout (KO) mice. These cells generally exhibited a regular spiking (RS) pattern, while there were significant increases in the number of cells that adopted intrinsic bursting (IB) compared with age-matched wild type (WT) cells. The cells subgrouped according to their firing patterns and maturation differed significantly in membrane and discharge properties between KO and WT. The changes in the intrinsic properties were consistent with highly facilitated discharges in KO cells induced by current injection. Spontaneous activities of RS neurons driven by local network were also increased in the KO cells, especially in neonate groups. Under an epileptiform condition mimicked by omission of Mg2+ in extracellular solution, these RS neurons from KO mice were more likely to switch to burst discharges. Analysis on bursts revealed that the KO cells tended to form burst discharges and even severe events manifested as seizure-like ictal discharges. These results suggest that alterations in intrinsic properties in individual neurons are involved in the abnormal excitability of cortical circuitry and possibly account for the pathogenesis of epilepsy in FXS. (C) 2016 Elsevier Inc. All rights reserved.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81271197]; Yangcheng Scholar Research Project of Guangzhou Municipal College [1201561584]; Science and Technology Project of Guangzhou [201508020011]
第一作者机构:[1]Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China[2]Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou, China[3]Department of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, China
通讯作者:
通讯机构:[*1]Institute of neuroscience and the second affiliated hospital of GuangzhouMedical University, Chang-gang-dong Road 250, Guangzhou 510260, China.
推荐引用方式(GB/T 7714):
Zhang Linming,Liang Zhanrong,Zhu Pingping,et al.Altered intrinsic properties and bursting activities of neurons in layer IV of somatosensory cortex from Fmr-1 knockout mice[J].EXPERIMENTAL NEUROLOGY.2016,280:60-69.doi:10.1016/j.expneurol.2016.03.025.
APA:
Zhang, Linming,Liang, Zhanrong,Zhu, Pingping,Li, Meng,Yi, Yong-Hong...&Su, Tao.(2016).Altered intrinsic properties and bursting activities of neurons in layer IV of somatosensory cortex from Fmr-1 knockout mice.EXPERIMENTAL NEUROLOGY,280,
MLA:
Zhang, Linming,et al."Altered intrinsic properties and bursting activities of neurons in layer IV of somatosensory cortex from Fmr-1 knockout mice".EXPERIMENTAL NEUROLOGY 280.(2016):60-69