Chondroitinase administration and pcDNA3.1-BDNF-BMSC transplantation promote motor functional recovery associated with NGF expression in spinal cord-transected rat
机构：[1]Department of Anesthesiology, Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, People’s Republic ofChina四川大学华西医院[2]Department of Neurosurgery, Institute of Neuroscience, No1. Affiliated hospital, Kunming Medical University, Kunming, People’s Republic of China外科科室神经外科神经外一科（神经外科）昆明医科大学附属第一医院[3]Department ofNeurosurgery, Shanghai Jiao Tong University Affiliated 6th People’s Hospital, Shanghai, People’s Republic of China
Study design: We evaluated whether combination of chondroitinase (chABC) administration and brain-derived neurotrophic factor (BDNF)-mesenchymal stem cell (MSC) transplantation could provide an optimal effect for the treatment of spinal cord injury (SCI) subjected to complete transection. Objectives: Behavioral assessments and DBA tracing were used to evaluate the effects of combination of chABC administration and BDNF-MSC transplantation on axonal regeneration and functional improvement in SCT rats. Setting: Sichuan, China Methods: Bone mesenchymal stem cells (BMSCs) were cultured and overexpressed BDNF recombinant vector was constructed into MSCs, then transplanted into the impaired spinal cord, together with chABC administration. Finally, the cortical spinal tract regeneration was detected by DBA tracing at 4 weeks post operation, and the expression of nerve growth factor (NGF), BDNF, neurotrophic factor (NT)-3, NT-4, fibroblast growth factor (FGF-2)-2, B cell lymphoma 2 (BCL-2) assaciated X protein (BAX) and BCL-2 in the caudal cord tissues was assessed by reverse transcription-PCR. Results: Animals received both BDNF-BMSC transplantation and chABC administration presented the best functional recovery and obvious axonal regeneration. Moreover, NGF expression was significantly higher than that in the other groups. Conclusion: Co-treated strategy could effectively promote motor functional recovery and axonal regeneration in SCT rats associated with NGF upregulation.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81271358, 81070991]; Natural Science Funding of Zhejiang Province [Y2090864]
第一作者机构：[1]Department of Anesthesiology, Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, People’s Republic ofChina
通讯作者：
通讯机构：[*1]Department of Anesthesiology, Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China[*2]Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6th People’s Hospital, Shanghai 200233, People’s Republic of China.
推荐引用方式(GB/T 7714)：
Xiong L-L,Li Y.,Shang F-F,et al.Chondroitinase administration and pcDNA3.1-BDNF-BMSC transplantation promote motor functional recovery associated with NGF expression in spinal cord-transected rat[J].SPINAL CORD.2016,54(12):1088-1095.doi:10.1038/sc.2016.55.
APA：
Xiong, L-L,Li, Y.,Shang, F-F,Chen, S-W,Chen, H....&Wang, X-Y.(2016).Chondroitinase administration and pcDNA3.1-BDNF-BMSC transplantation promote motor functional recovery associated with NGF expression in spinal cord-transected rat.SPINAL CORD,54,(12)
MLA：
Xiong, L-L,et al."Chondroitinase administration and pcDNA3.1-BDNF-BMSC transplantation promote motor functional recovery associated with NGF expression in spinal cord-transected rat".SPINAL CORD 54..12(2016):1088-1095
