机构：[1]Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China,[2]Department of Biochemistry & Molecular Biology, School of Basic Medicine, Dali University, Dali, China,[3]Department of Rheumatology & Immunology, First Affiliated Hospital of Kunming Medical University, Kunming, China,内科科室风湿免疫科昆明医科大学附属第一医院[4]Department of Hepatobiliary Surgery, Second Affiliated Hospital of Kunming Medical University, Kunming, China
Chemokines are small secreted proteins functionally involved in the immune system's regulation of lymphocyte migration across numerous mammalian species. Given its growing popularity in immunological models, we investigated the structure and function of chemokine CXCL12 protein in tree shrews. We found that CXCL12 and its receptor CXCR4 in tree shrew had structural similarities to their homologous human proteins. Phylogenetic analysis supports the view that tree shrew is evolutionarily-close to the primates. Our results also showed that the human recombinant CXCL12 protein directly enhanced the migration of tree shrew's lymphocytes in vitro, while AMD3100 enhanced the mobilization of hematopoietic progenitor cells (HPCs) from bone marrow into peripheral blood in tree shrew in vivo. Collectively, these findings suggested that chemokines in tree shrews may play the same or similar roles as those in humans, and that the tree shrew is a viable animal model for studying human immunological diseases.
基金：
National Natural Science Foundation of China (No. 81271330, No . 31160237, and No . 31260276), Yunnan
Province Science and Technology Innovation Team (No . 2011CI123), Talent Program of Yunnan Province (No . W8110305), Foundation of School of Life Sciences
of Yunnan University (No . 2012S301), and the Natural Science Foundation of Yunnan Province (No . 2011BC003). The funders had no roles in study design, data
collection and analysis, decision to publish or preparation of the manuscript.
第一作者机构：[1]Department of Biochemistry & Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China,[2]Department of Biochemistry & Molecular Biology, School of Basic Medicine, Dali University, Dali, China,
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Chen Guiyuan,Wang Wei,Meng Shengke,et al.CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function[J].PLOS ONE.2014,9(5):doi:10.1371/journal.pone.0098231.
APA：
Chen, Guiyuan,Wang, Wei,Meng, Shengke,Zhang, Lichao,Wang, Wenxue...&Li, Meizhang.(2014).CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function.PLOS ONE,9,(5)
MLA：
Chen, Guiyuan,et al."CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function".PLOS ONE 9..5(2014)
