机构:[1]Department of Emergency, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032内科科室急诊医学科昆明医科大学附属第一医院[2]Surgical Intensive Care Unit, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650106, P.R. China
RAW264.7 cells are one of the major sources of productive inflammatory biomediators, including tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6. TNF-alpha-induced protein 8-like 2 (TIPE2) is an essential negative regulator of Toll-like and T-cell receptors, and the selective expression in the immune system prevents hyper-responsiveness and maintains immune homeostasis. The aim of the present study was to investigate whether atorvastatin upregulates the expression of TIPE2 and further regulates the inflammatory response and oxidation emergency response in RAW264.7 cells. RAW264.7 cells were incubated in Dulbecco's modified Eagle's medium containing lipopolysaccharide (LPS) in the presence or absence of atorvastatin. Following incubation, the medium was collected and the levels of TNF-alpha and IL-6 were measured using an enzyme-linked immunosorbent assay. The cells were harvested, and the mRNA and protein expression levels of TIPE2, macrophage migration inhibitory factor (MIF), fa and nuclear factor (NF-kappa B)-kappa B were analysed using quantitative polymerase chain reaction and western blotting analysis, respectively, the expression of NOS, COX-2 and HO-1 protein were essayed by western blotting analysis, NO and ROS activities were determined. The results revealed that LPS increased the mRNA and protein expression levels of TIPE2, MIF and NF-kappa B, as well as the production of IL-6 and TNF-alpha, in a dose and time dependent manner in RAW264.7 cells. Meanwhile, LPS enhanced the expression of NOS and COX-2 protein, blocked HO-1 protein expression, increased NO and PGE2 production and ROS activity in a dose dependent manner in RAW264.7 cells. Atorvastatin significantly increased LPS induced expression of TIPE2, downregulated the expression of NOS, COX-2, MIF and NF-kappa B and the production of PGE2, NO, IL-6 and TNF-alpha in a time and dose dependent manner, and increased HO-1 protein expression, reduced ROS production in a dose dependent manner. The observations indicated that atorvastatin upregulated LPS induced expression of TIPE2 and consequently inhibited MIF, NF-kappa B, NOS and COX-2 expression and the production of NO, PGE2, TNF-alpha and IL-6, increased HO-1 expression, and inhibited ROS activity in cultured RAW264.7 cells.
第一作者机构:[1]Department of Emergency, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032[*1]Department of Emergency, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, Yunan 650000, P.R. China
通讯作者:
通讯机构:[*1]Department of Emergency, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, Yunan 650000, P.R. China
推荐引用方式(GB/T 7714):
Liu Ming-Wei,Su Mei-Xian,Zhang Wei,et al.Atorvastatin increases lipopolysaccharide-induced expression of tumour necrosis factor-a-induced protein 8-like 2 in RAW264.7 cells[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2014,8(1):219-228.doi:10.3892/etm.2014.1722.
APA:
Liu, Ming-Wei,Su, Mei-Xian,Zhang, Wei,Wang, Li&Qian, Chuan-Yun.(2014).Atorvastatin increases lipopolysaccharide-induced expression of tumour necrosis factor-a-induced protein 8-like 2 in RAW264.7 cells.EXPERIMENTAL AND THERAPEUTIC MEDICINE,8,(1)
MLA:
Liu, Ming-Wei,et al."Atorvastatin increases lipopolysaccharide-induced expression of tumour necrosis factor-a-induced protein 8-like 2 in RAW264.7 cells".EXPERIMENTAL AND THERAPEUTIC MEDICINE 8..1(2014):219-228