机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming,Yunnan, China[2]First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China昆明医科大学附属第一医院[3]Yunnan Institute of Materia Medica, Kunming, Yunnan, China
The Tongshu Capsule (TSC) is a prevalent form of traditional Chinese medicine widely used for its purported effects in treating mammary gland hyperplasia and inflammation. Though successful in several clinical studies, there is no clear evidence as to why TSC has a positive treatment effect, and little known about underlying mechanism that may account for it. In this study, we examined the effects of TSC and found that it has a comparatively strong growth inhibition on ER alpha positive breast cancer cells. TSC seems to cause G1 cell cycle arrest instead of apoptosis. Interestingly, TSC also down-regulated the expression of ER alpha and Cyclin D1. Consistently, TSC suppressed E2 mediated ER alpha downstream gene expression and cell proliferation in ER alpha positive breast cancer cell lines MCF7 and T47D. Depletion of ER alpha partially abolished the effects of TSC on the decrease of Cyclin D1 and cell viability. Our findings suggest that TSC may have therapeutic effects on ER alpha positive breast cancers and moreover that TSC may suppress breast epithelial cell proliferation by inhibiting the estrogen pathway.
基金：
This study was partially funded by Yunnan Institute of Materia Medica, which is the manufacturer of Tongshu Capsule. This funder provided support in
the form of salaries for author JW, but did not have any additional role in the study design, data collection and analysis, decision to publish, orpreparation of the
manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section. This study was also supported in part by National Nature
Science Foundation of China (81120108019, U1132605, and 81325016), and a grant from the Yunnan Province High-Profile Scientist Program (2010CI114). These
funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
第一作者机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming,Yunnan, China
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通讯作者：
推荐引用方式(GB/T 7714)：
Du Chengzhi,Li Zhen,Wang Shuang,et al.Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor alpha and Suppresses Human Breast Cancer Cell Proliferation[J].PLOS ONE.2014,9(8):doi:10.1371/journal.pone.0104261.
APA：
Du, Chengzhi,Li, Zhen,Wang, Shuang,Zhou, Zhongmei,Wang, Jingkun...&Chen, Ceshi.(2014).Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor alpha and Suppresses Human Breast Cancer Cell Proliferation.PLOS ONE,9,(8)
MLA：
Du, Chengzhi,et al."Tongshu Capsule Down-Regulates the Expression of Estrogen Receptor alpha and Suppresses Human Breast Cancer Cell Proliferation".PLOS ONE 9..8(2014)
