机构:[1]Department of Histology and Embryology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, People’s Republic of China[2]Minimally Invasive Neurosurgery, First Affiliated Hospital of Kunming Medical University, 295 Xi Chang Road, Kunming 650032, People’s Republic of China外科科室神经外科神经外一科(神经外科)昆明医科大学附属第一医院
Expression of angiotensin II (Ang II) and its receptors (AT(1)/AT(2)) is undetected in the mature microglia in normal brain. We report here that the immunoexpression of Ang II and AT(1)/AT(2) was altered in activated microglia notably at 1 week in rats subjected to middle cerebral artery occlusion (MCAO). Immunolabeled activated microglia were widely distributed in the infarcted cerebral tissue after MCAO. By enzyme immunoassay, Ang II protein expression levels of the ischemic tissues were decreased drastically at 12 h after ischemia, then rose rapidly at 3 days and 1 week after MCAO when compared with the control. On the other hand, AT(1) and AT(2) receptor mRNA and protein levels were up-regulated after MCAO, peaking at 12 h, but declined thereafter. Expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) mRNA and protein levels was concomitantly increased. Edaravone significantly suppressed Ang II and AT(1)/AT(2) receptor expression as well as that of TNF-alpha and IL-1 beta suggesting that microglia-derived Ang II can act through an autocrine manner via its receptor that may be linked partly to the production of proinflammatory cytokines. We conclude that neuroinflammation in MCAO may be attenuated by Edaravone which acts through suppression of expression of Ang II and its receptors and proinflammatory cytokines in activated microglia.
基金:
National Natural Science Research Grants of China [30900778, 31260254]
第一作者机构:[1]Department of Histology and Embryology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, People’s Republic of China
通讯作者:
推荐引用方式(GB/T 7714):
Wu Chun-Yun,Zha Hao,Xia Qing-Qing,et al.Expression of angiotensin II and its receptors in activated microglia in experimentally induced cerebral ischemia in the adult rats[J].MOLECULAR AND CELLULAR BIOCHEMISTRY.2013,382(1-2):47-58.doi:10.1007/s11010-013-1717-4.
APA:
Wu, Chun-Yun,Zha, Hao,Xia, Qing-Qing,Yuan, Yun,Liang, Xiao-Yan...&Li, Juan-Juan.(2013).Expression of angiotensin II and its receptors in activated microglia in experimentally induced cerebral ischemia in the adult rats.MOLECULAR AND CELLULAR BIOCHEMISTRY,382,(1-2)
MLA:
Wu, Chun-Yun,et al."Expression of angiotensin II and its receptors in activated microglia in experimentally induced cerebral ischemia in the adult rats".MOLECULAR AND CELLULAR BIOCHEMISTRY 382..1-2(2013):47-58