机构:[1]Department of Radiology, West China Hospital of Sichuan University, Chengdu四川大学华西医院[2]Department of Diagnostic Ultrasound, Second University Hospital of Sichuan University, Chengdu[3]Department of Nuclear Medicine, First Hospital of Kunming Medical University, Kunming内科科室核医学科昆明医科大学附属第一医院[4]National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China
To develop a molecular probe for MRI detection of human tumor telomerase reverse transcriptase (hTERT) mRNA expression. Uniformly phosphorothioate-modified hTERT antisense oligonucleotide (ASON) homing hTERT mRNA was labeled with gadolinium (Gd) through the bifunctional chelator 1,4,7, 10-tetraazacyclododecane-N, N', N'', N'''-tetraacetic acid (DOTA) stirred within 45 minutes at 60 degrees C. The Gd labeled probes were characterized in vitro. The cellular uptake rate and biodistribution of 99mTc-DOTA-ASON was measured instead of that of Gd-DOTA-ASON. A549 lung adenocarcinoma model was established in BALB/c nude mice and Gd-DOTA-ASON was injected intraperitoneally and MR images were acquired using 7.0T Micro-MRI (Bruker Biospec, Ettlingen, Germany) at different time points. Immunohistochemical analysis of telomerase activity of each xenograft was operated two days after in vivo imaging. The binding efficiency of Gd-DOTA-ASON reached as high as 71.7 +/- 4.5% (n = 6). Gd-DOTA-ASON displayed perfect stability in fresh human serum at 37 degrees C for 24 h. Compared with normal lung cells, A549 cells showed an obviously higher 2.6%, P < 0.05). The signal intensity of A549 xenografts can be enhanced by Gd-DOTA-ASON and the signal to noise ratio (SNR) of tumor to muscle reached 2.37 and maintained a relatively high level within 6 h after injection. The activity of hTERT in A549 tumors can be suppressed by Gd-DOTA-ASON in pathological slices. The results of this study show that Gd-DOTA-ASON can be a promising intracellular MR contrast probe for targeting telomerase-positive carcinomas. (Cancer Sci 2012; 103: 14341439)