机构:[1]Clinical Pharmacy and Pharmacology Research Institute, Second Xiangya Hospital, Central South University, Changsha 410011, People’s Republic of China[2]Center of Clinical Pharmacy, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, People’s Republic of China医技科室医学影像中心药剂科CT室昆明医科大学附属第一医院[3]School of Chemistry, Xiangtan University, Xiangtan 411105, People’s Republic of China
A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method coupled with column-switching technique was developed for the determination of olanzapine in rat brain microdialysates. A C8 guard column was used to desalt the samples before analytical separation on a C18 column and detection with tandem mass spectrometry. The mobile phase consisted of methanol/acetonitrile/water (v/v/v, 22.5/22.2/55) was used for desalting and the mobile phase consisted of methanol/acetonitrile/water (v/v/v, 43/43/14) was for analytical separation, water in both mobile phases contained 0.1% ammonium acetate. The lower limit of quantification (LLOQ) for olanzapine was 0.085 ng/ml. The method was linear from LLOQ to 34 ng/ml with a coefficient of determination >0.998. Intra- and inter-day accuracy and precision were determined with variability Less than 13.24% (R.S.D). This sensitive method was successfully applied to quantify the concentration of olanzapine in rat brain microdialysates. With this study, the effect of the alcohol extract of Schisandra sphenanthera Rehd. et Wils on the concentration of olanzapine in brain was investigated. (C) 2012 Elsevier B.V. All rights reserved.
语种:
外文
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2013]版:
大类|3 区医学
小类|3 区生化研究方法3 区分析化学
最新[2023]版:
大类|3 区医学
小类|3 区生化研究方法3 区分析化学
JCR分区:
出版当年[2012]版:
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICAL
最新[2023]版:
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICAL
第一作者机构:[2]Center of Clinical Pharmacy, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, People’s Republic of China
通讯作者:
推荐引用方式(GB/T 7714):
Zheng Qiaoling,Wang Feng,Li Huande,et al.Quantitative analysis of olanzapine in rat brain microdialysates by HPLC-MS/MS coupled with column-switching technique[J].JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES.2012,905:127-132.doi:10.1016/j.jchromb.2012.07.024.
APA:
Zheng, Qiaoling,Wang, Feng,Li, Huande,Xu, Ping,Tang, Huaibo...&Cheng, Rihua.(2012).Quantitative analysis of olanzapine in rat brain microdialysates by HPLC-MS/MS coupled with column-switching technique.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,905,
MLA:
Zheng, Qiaoling,et al."Quantitative analysis of olanzapine in rat brain microdialysates by HPLC-MS/MS coupled with column-switching technique".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 905.(2012):127-132