机构:[1]Department of Pathology, The First Affiliated Hospital of Kunming Medical College, 650032 Kunming, China医技科室病理科昆明医科大学附属第一医院[2]Department of Dental Research, The Affiliated Stomatology Hospital of Kunming Medical College, 650031 Kunming, China昆明医科大学附属第一医院[3]Department of Pathology, Kunming Medical College, 650031 Kunming, China
Tumor-stroma interactions play a significant role in tumor development and progression. Our study employed an in vitro co-culture model of epithelial cells and fibroblasts to investigate the mechanism of and interaction between lung epithelial cell transformation and fibroblast activation induced by Yunnan tin mine dust. Epithelial cell transformation was evaluated using concanavalin A agglutination and anchorage-independent growth assays, and fibroblast activation was assessed via immunohistochemistry. The TGF-beta 1/Smad pathway was monitored by Western blot analysis and ELISA. We found concanavalin A agglutination and anchorage-independent growth assays of dust-exposed epithelial cells were positive, dust-exposed fibroblasts expressed alpha-SMA, and during the mine dust-induced tumorigenesis, TGF-beta 1/Smad signaling pathway changed. In conclusion, Yunnan tin mine dust is able to induce the malignant transformation of bronchial epithelial cells and fibroblast activation. Epithelial cells are the main target of mine dust. Bronchial epithelial cell transformation and fibroblast activation are correlated and synergistic. Their interdependence is related to the TGF-beta 1/Smad signaling pathway.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30560053]; Department of Science and Technology of Yunnan Province, Kunming Medical College Collaborative Special Project [2008 CD009]; Ministry of Education [NCET-07-0388, 20050678004]
第一作者机构:[1]Department of Pathology, The First Affiliated Hospital of Kunming Medical College, 650032 Kunming, China
共同第一作者:
通讯作者:
推荐引用方式(GB/T 7714):
Bian Li,He Yong-Wen,Tang Rui-Zhu,et al.Induction of lung epithelial cell transformation and fibroblast activation by Yunnan tin mine dust and their interaction[J].MEDICAL ONCOLOGY.2011,28:S560-S569.doi:10.1007/s12032-010-9655-4.
APA:
Bian, Li,He, Yong-Wen,Tang, Rui-Zhu,Ma, Li-Ju,Wang, Chun-Yan...&Jin, Ke-Wei.(2011).Induction of lung epithelial cell transformation and fibroblast activation by Yunnan tin mine dust and their interaction.MEDICAL ONCOLOGY,28,
MLA:
Bian, Li,et al."Induction of lung epithelial cell transformation and fibroblast activation by Yunnan tin mine dust and their interaction".MEDICAL ONCOLOGY 28.(2011):S560-S569