Co-expression of CD173 (H2) and CD174 (Lewis Y) with CD44 suggests that fucosylated histo-blood group antigens are markers of breast cancer-initiating cells
机构：[1]Laboratory of Molecular and Experimental Pathology, Key Laboratory of Animal Models and Human Disease Mechanisms of CAS and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan 650223, China[2]Berlin-Buch, Germany[3]Department of General Surgery,First Affiliated Hospital of Kunming Medical College,Kunming,China外科科室昆明医科大学附属第一医院[4]College of Life Sciences, Longyan University, Longyan, China
Histo-blood group antigens CD173 (H2) and CD174 (Lewis Y) are known to be developmentally regulated carbohydrate antigens which are expressed to a varying degree on many human carcinomas. We hypothesized that they might represent markers of cancer-initiating cells (or cancer stem cells, CSC). In order to test this hypothesis, we examined the co-expression of CD173 and CD174 with stem cell markers CD44 and CD133 by flow cytometry analysis, immunocytochemistry, and immunohistochemistry on cell lines and tissue sections from breast cancer. In three breast cancer cell lines, the percentage of CD173(+)/CD44(+) cells ranged from 17% to > 60% and of CD174(+)/CD44(+) from 21% to 57%. In breast cancer tissue sections from 15 patients, up to 50% of tumor cells simultaneously expressed CD173, CD174, and CD44 antigens. Co-expression of CD173 and CD174 with CD133 was also observed, but to a lesser percentage. Co-immunoprecipitation and sandwich ELISA experiments on breast cancer cell lines suggested that CD173 and CD174 are carried on the CD44 molecule. The results show that in these tissues CD173 (H2) and CD174 (LeY) are associated with CD44 expression, suggesting that these carbohydrate antigens are markers of cancer-initiating cells or of early progenitors of breast carcinomas.
基金：
Yunnan Province Science and Technology DepartmentNatural Science Foundation of Yunnan Province [2008CCO15]
第一作者机构：[1]Laboratory of Molecular and Experimental Pathology, Key Laboratory of Animal Models and Human Disease Mechanisms of CAS and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan 650223, China[4]College of Life Sciences, Longyan University, Longyan, China
通讯作者：
推荐引用方式(GB/T 7714)：
Lin Wei-Ming,Karsten Uwe,Goletz Steffen,et al.Co-expression of CD173 (H2) and CD174 (Lewis Y) with CD44 suggests that fucosylated histo-blood group antigens are markers of breast cancer-initiating cells[J].VIRCHOWS ARCHIV.2010,456(4):403-409.doi:10.1007/s00428-010-0897-5.
APA：
Lin, Wei-Ming,Karsten, Uwe,Goletz, Steffen,Cheng, Ruo-Chuan&Cao, Yi.(2010).Co-expression of CD173 (H2) and CD174 (Lewis Y) with CD44 suggests that fucosylated histo-blood group antigens are markers of breast cancer-initiating cells.VIRCHOWS ARCHIV,456,(4)
MLA：
Lin, Wei-Ming,et al."Co-expression of CD173 (H2) and CD174 (Lewis Y) with CD44 suggests that fucosylated histo-blood group antigens are markers of breast cancer-initiating cells".VIRCHOWS ARCHIV 456..4(2010):403-409
