机构:[1]Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan 650091, People’s Republic of China[2]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, People’s Republic of China内科科室心脏内科昆明医科大学附属第一医院[3]Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, People’s Republic of China医技科室医学影像中心CT室昆明医科大学附属第一医院云南省第一人民医院
PaaX is a global regulator of the phenylacetyl-coenzyme A catabolon that adjusts the expression of different operons to that of the paa-encoded central pathway. In this study, the PaaX-like protein from the hyperthermophilic archaeon Sulfolobus solfataricus P2 was successfully crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. Diffraction data were obtained to a resolution of 3.0 angstrom using synchrotron radiation at the Photon Factory. The crystal belonged to space group P321, with unit-cell parameters a = 86.4, b = 86.4, c = 105.5 angstrom.
基金:
NSFCNational Natural Science Foundation of China [30860278, 2006C0071M, 2007C0001R]; First Affiliated Hospital of Kunming Medical College [2006bs08]; [2007AA021303]; [2007CB411600]
语种:
外文
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2010]版:
无
最新[2023]版:
大类|4 区生物学
小类|4 区生化研究方法4 区生化与分子生物学4 区生物物理4 区晶体学
JCR分区:
出版当年[2009]版:
Q4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4BIOPHYSICSQ4CRYSTALLOGRAPHYQ4BIOCHEMICAL RESEARCH METHODS
最新[2023]版:
Q3CRYSTALLOGRAPHYQ4BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4BIOPHYSICS
第一作者机构:[1]Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan 650091, People’s Republic of China[2]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, People’s Republic of China
共同第一作者:
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推荐引用方式(GB/T 7714):
Cao Yi,Lou Zhiyong,Sun Yuna,et al.Crystallization and preliminary X-ray characterization of a PaaX-like protein from Sulfolobus solfataricus P2[J].ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS.2009,65:776-778.doi:10.1107/S174430910902404X.
APA:
Cao, Yi,Lou, Zhiyong,Sun, Yuna,Xue, Fei,Feng, Changzeng...&Zhang, Keqin.(2009).Crystallization and preliminary X-ray characterization of a PaaX-like protein from Sulfolobus solfataricus P2.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,65,
MLA:
Cao, Yi,et al."Crystallization and preliminary X-ray characterization of a PaaX-like protein from Sulfolobus solfataricus P2".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 65.(2009):776-778