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Crystallization and preliminary X-ray characterization of a PaaX-like protein from Sulfolobus solfataricus P2

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机构: [1]Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan 650091, People’s Republic of China [2]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, People’s Republic of China [3]Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, People’s Republic of China
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PaaX is a global regulator of the phenylacetyl-coenzyme A catabolon that adjusts the expression of different operons to that of the paa-encoded central pathway. In this study, the PaaX-like protein from the hyperthermophilic archaeon Sulfolobus solfataricus P2 was successfully crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. Diffraction data were obtained to a resolution of 3.0 angstrom using synchrotron radiation at the Photon Factory. The crystal belonged to space group P321, with unit-cell parameters a = 86.4, b = 86.4, c = 105.5 angstrom.

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大类 | 4 区 生物学
小类 | 4 区 生化研究方法 4 区 生化与分子生物学 4 区 生物物理 4 区 晶体学
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出版当年[2009]版:
Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Q4 BIOPHYSICS Q4 CRYSTALLOGRAPHY Q4 BIOCHEMICAL RESEARCH METHODS
最新[2023]版:
Q3 CRYSTALLOGRAPHY Q4 BIOCHEMICAL RESEARCH METHODS Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Q4 BIOPHYSICS

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第一作者机构: [1]Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan 650091, People’s Republic of China [2]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, People’s Republic of China
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