机构:[1]Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China[2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China[3]Kunming Medical College, Kunming, Yunnan 650032, China[4]Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650031, China外科科室麻醉手术科(医技)昆明医科大学附属第一医院云南省第一人民医院
Snake venom Kunitz/BPTI members are good tools for understanding of structure-functional relationship between serine proteases and their inhibitors. A novel dual Kunitz/BPTI serine proteinase inhibitor named OH-TCI (trypsin- and chymotrypsin-dual inhibitor from Ophiophagus hannah) was isolated from king cobra venom by three chromatographic steps of gel filtration, trypsin affinity and reverse phase HPLC. OH-TCI is composed of 58 amino acid residues with a molecular mass of 6339 Da. Successful expression of OH-TCI was performed as the maltose-binding fusion protein in E. coli DH5 alpha . Much different from Oh11-1, the purified native and recombinant OH-TCI both had strong inhibitory activities against trypsin and chymotrypsin although the sequence identity (74.1%) between them is very high. The inhibitor constants (K-i) of recombinant OH-TCI were 3.91 x 10(-7) and 8.46 x 10(-8) M for trypsin and chymotrypsin, respectively. To our knowledge, it was the first report of Kunitz/BPTI serine proteinase inhibitor from snake venom that had equivalent trypsin and chymotrypsin inhibitory activities. (C) 2008 Elsevier Inc. All rights reserved.
基金:
Chinese National Natural Science FoundationNational Natural Science Foundation of China [30630014, 30570359, 30670412]; Chinese Academy of SciencesChinese Academy of Sciences [KSCX2-YW-R-088]
第一作者机构:[1]Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China[2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
共同第一作者:
通讯作者:
通讯机构:[*1]Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China.
推荐引用方式(GB/T 7714):
He Ying-Ying,Liu Shu-Bai,Lee Wen-Hui,et al.Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom[J].PEPTIDES.2008,29(10):1692-1699.doi:10.1016/j.peptides.2008.05.025.
APA:
He, Ying-Ying,Liu, Shu-Bai,Lee, Wen-Hui,Qian, Jin-Qiao&Zhang, Yun.(2008).Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom.PEPTIDES,29,(10)
MLA:
He, Ying-Ying,et al."Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom".PEPTIDES 29..10(2008):1692-1699