机构:[1]Institute of Basic Medical Sciences and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China.[2]State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, P.R. China.[3]Laboratory of Hematology, Hematology and BMT Center, Department of Internal Medicine and Biomedical Science, University of Parma, Parma, Italy.[4]Department of Hematology, Second Affiliated Hospital of Soochow University, Suzhou, P.R. China.苏州大学附属第二医院[5]Department of Hematology, First Affiliated Hospital of Kunming Medical College, Kunming, P.R. China.内科科室血液内科昆明医科大学附属第一医院
One of the clinical features of multiple myeloma (MM) is the occurrence of skeletal events, which are characterized by increased bone resorption and decreased bone formation. In contrast to enhanced osteoclastogenesis, little is known about the mechanism of impaired bone formation in MM. Because TAZ, a Runx2/Cbfa1 transcriptional co-activator, has recently been shown to modulate mesenchymal stem cell (MSC) differentiation in favor of osteoblast differentiation, we investigated whether the regulation of TAZ expression played a role in the decreased bone formation of MM. We isolated and purified Flk-1(+)CD31(-)CD34(-) cells with MSC characters from bone marrow (BM) of myeloma patients and healthy donors. We found the osteogenic potential of the MSCs from myeloma patients decreased significantly, and TAZ expression of these cells was lower than that of healthy donors. Human myeloma cell lines (HMCLs) and CD138(+) myeloma cells (MCs) from myeloma patients inhibited osteogenesis of the MSCs from healthy volunteers, which were accompanied by a reduced TAZ expression and elevated TNF-alpha concentration in the supernatant of co-culture systems. The repressed osteogenesis and TAZ expression were both partially restored by neutralization of TNF-alpha. Thus, the decreased osteogenic potential of MSCs of myeloma patients was in part due to TNF-alpha suppressed TAZ expression.
基金:
This work was supported by grants from the “863 Projects” of the Ministry of Science and Technology of the
P.R. of China (no. 2002AA205061); the China Medical
Board of New York, Stem Cell Biology, Engineering
(Grant no. 01-748); the National Natural Science Foundation of China (no. 30570771, no. 30125018); the National Key Project for Basic Research of China (no.
2001CB509907); and the Cheung Kong Scholars program. R.C.H.Z. is a Cheung Kong Scholar in the P.R. of
China.
语种:
外文
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2008]版:
大类|3 区医学
小类|3 区血液学3 区医学:研究与实验3 区移植
最新[2023]版:
大类|3 区医学
小类|3 区移植4 区细胞与组织工程4 区血液学4 区医学:研究与实验
JCR分区:
出版当年[2007]版:
Q2TRANSPLANTATIONQ2HEMATOLOGYQ2MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版:
Q2HEMATOLOGYQ2TRANSPLANTATIONQ3CELL & TISSUE ENGINEERINGQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构:[1]Institute of Basic Medical Sciences and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China.[4]Department of Hematology, Second Affiliated Hospital of Soochow University, Suzhou, P.R. China.
共同第一作者:
通讯作者:
通讯机构:[*1]Institute of Basic Medical Sciences and School of Basic Medicine Director of Center for Tissue Engineering Chinese Academy of Medical Sciences and Peking Union Medical College Dongdansantiao No. 5 Beijing 100005, P.R. China
推荐引用方式(GB/T 7714):
BINGZONG LI,MINGXIA SHI,JING LI,et al.Elevated tumor necrosis factor-alpha suppresses TAZ expression and impairs steogenic potential of Flk-1(+) mesenchymal stem cells in patients with multiple myeloma[J].STEM CELLS AND DEVELOPMENT.2007,16(6):921-930.doi:10.1089/scd.2007.0074.
APA:
BINGZONG LI,MINGXIA SHI,JING LI,HONGBING ZHANG,BIN CHEN...&ROBERT CHUNHUA ZHAO.(2007).Elevated tumor necrosis factor-alpha suppresses TAZ expression and impairs steogenic potential of Flk-1(+) mesenchymal stem cells in patients with multiple myeloma.STEM CELLS AND DEVELOPMENT,16,(6)
MLA:
BINGZONG LI,et al."Elevated tumor necrosis factor-alpha suppresses TAZ expression and impairs steogenic potential of Flk-1(+) mesenchymal stem cells in patients with multiple myeloma".STEM CELLS AND DEVELOPMENT 16..6(2007):921-930