机构：[1]Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China外科科室骨科昆明医科大学附属第一医院
出处：
ISSN：
摘要：
Abstract
BACKGROUND: Prolyl hydroxylase inhibitors can stabilize hypoxia inducible factor to up-regulate downstream genes
expression, thereby regulating cell apoptosis.
OBJECTIVE: To evaluate the effects of the prolyl hydroxylase inhibitors dimethyloxalglycine (DMOG) on apoptosis of
bone marrow-derived mesenchymal stem cells (BMSCs) induced by serum deprivation.
METHODS: Bone marrow mononuclear cells were harvested from Kunming mice bone marrow cavity of the femur and
tibia and subcultured. The passage five mononuclear cells were identified by flow cytometry and tri-lineage differentiation.
Bone marrow mononuclear cells were cultured in serum-free DMEM for serum deprivation (serum-deprivation group) and
DMEM containing 10% fetal bovine serum (normal control group) for 24, 48, and 72 hours. Experimental group was
treated with 1 mmol/L DMOG based on serum-free culture.
RESULTS AND CONCLUSION: The bone marrow mononuclear cells from the mice were purified through subculture and
were verified as BMSCs through cell surface marker identification by flow cytometry. Compared with serum-deprivation
group, the apoptosis rate was significantly decreased in experimental group (P < 0.01). Results indicate that DMOG can
suppress BMSCs apoptosis induced by serum deprivation.
语种：
中文
第一作者：
第一作者机构：[1]Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
通讯作者：
通讯机构：[1]Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China[*1]Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
