机构：[a]Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China[b]Department of Anesthesiology and ICU, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China[c]Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, China外科科室麻醉手术科（医技）昆明医科大学附属第一医院
BACKGROUND: Reduced B cell numbers play a critical role in sepsis immunosuppression. The role of B-cell maturation regulated by T follicular helper (Tfh) cells in reduced B cell numbers during sepsis remains unclear. We tested the hypothesis that impaired B-cell maturation contributes to reduced B cell numbers. DESIGN: Retrospective study and observational prospective cohort study. SETTINGS: Critical care units. METHODS: To identify the exact lymphocyte counts that affect the prognosis of sepsis, we first conducted a retrospective study. Then in the prospective cohort study, differences in B-cell maturation, B cell death, and numbers of circulating Tfh (cTfh) cell were compared between 28-day survivors and 28-day non-survivors, mainly by flow cytometry and enzyme-linked immunosorbent assay. MAIN RESULTS: In retrospective study (n = 123), we found patients with lymphocyte counts less than 0.4 × 10 cells/L had higher mortality than patients with lymphocyte counts above 0.4 × 10 cells/L. In observational prospective cohort study (n = 40), compared with survivors, non-survivors had fewer numbers of mature B cell and circulating Tfh (cTfh) cell (sepsis onset: memory B cells: 3.44% vs. 4.48%, antibody-secreting cells: 4.53% vs. 6.30%, cTfh cells: 3.57% vs. 4.49%; 24 h after sepsis onset: memory B cells: 4.05% vs. 7.20%, antibody-secreting cells: 5.25% vs. 8.78%, cTfh cells: 3.98% vs. 6.15%), while there were no differences in cell death of mature B cells between them. We further noticed the numbers of cTfh cell positively correlated with the numbers of mature B cell and immunoglobulin concentrations. CONCLUSIONS: Impaired B-cell maturation contributes to reduced B cell numbers, while the numbers of cTfh cell, acting as a warning indicator for sepsis prognosis, may be a new therapeutic target for treating sepsis.
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|3 区医学
小类|3 区血液学3 区外周血管病3 区外科4 区危重病医学
最新[2023]版：
大类|3 区医学
小类|3 区血液学3 区外周血管病3 区外科4 区危重病医学
JCR分区：
出版当年[2020]版：
Q2HEMATOLOGYQ2PERIPHERAL VASCULAR DISEASEQ2CRITICAL CARE MEDICINEQ2SURGERY
最新[2023]版：
Q1SURGERYQ2CRITICAL CARE MEDICINEQ2HEMATOLOGYQ2PERIPHERAL VASCULAR DISEASE
第一作者机构：[a]Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China[b]Department of Anesthesiology and ICU, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
推荐引用方式(GB/T 7714)：
Duan S,Jiao Y,Wang J,et al.Impaired B-Cell Maturation Contributes to Reduced B Cell Numbers and Poor Prognosis in Sepsis[J].SHOCK.2020,54(1):70-77.doi:10.1097/SHK.0000000000001478.
APA：
Duan, S,Jiao, Y,Wang, J,Tang, D,Xu, S...&Yu, W.(2020).Impaired B-Cell Maturation Contributes to Reduced B Cell Numbers and Poor Prognosis in Sepsis.SHOCK,54,(1)
MLA：
Duan, S,et al."Impaired B-Cell Maturation Contributes to Reduced B Cell Numbers and Poor Prognosis in Sepsis".SHOCK 54..1(2020):70-77
