机构:[1]Department of Hematology, The 920 Hospital of People's Liberation Army, Kunming, Yunnan, People's Republic of China.[2]Department of Rheumatology and Immunology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.内科科室风湿免疫科
Objective:
TP73-AS1 has been reported as an overexpressed oncogenic lncRNA in several types of cancer. However, these analyses of The Cancer Genome Atlas data set revealed downregulation of TP73-AS1 in acute myeloid leukemia (AML). In this study, we aimed to study the molecular mechanism between TP73-AS1 and cell proliferation in AML.
Methods:
Bone marrow (BM) samples were obtained from 50 AML patients and 50 healthy controls. Cell transient transfections were performed to analyze gene interactions. Dual-luciferase reporter assay, quantitative polymerase chain reaction and western blot were used to study the gene expressions. Cell proliferation was analyzed by CCK-8 method.
Results:
TP73-AS1 was confirmed to be downregulated in AML. TP73-AS1 was predicted to interact with miR-21, while overexpression of TP73-AS1 and miR-21 did not affect the expression of each other. Instead, overexpression of TP73-AS1 led to the upregulation of phosphatase and tensin homologue (PTEN), a downstream target of miR-21. Cell proliferation analysis showed that overexpression of TP73-AS1 and PTEN led to a decreased proliferation rate of AML cells. Overexpression of miR-21 played an opposite role and reduced the effects of overexpressing TP73-AS1 and PTEN.
Conclusion:
TP73-AS1 may regulate the miR-21/PTEN axis to affect cell proliferation in AML.
基金:
否
语种:
外文
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2022]版:
大类|4 区医学
小类|4 区医学:研究与实验4 区核医学4 区药学4 区肿瘤学
最新[2023]版:
大类|4 区医学
小类|4 区医学:研究与实验4 区肿瘤学4 区药学4 区核医学
JCR分区:
出版当年[2021]版:
Q2PHARMACOLOGY & PHARMACYQ2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3MEDICINE, RESEARCH & EXPERIMENTALQ3ONCOLOGY
最新[2023]版:
Q2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3MEDICINE, RESEARCH & EXPERIMENTALQ3ONCOLOGYQ3PHARMACOLOGY & PHARMACY
第一作者机构:[1]Department of Hematology, The 920 Hospital of People's Liberation Army, Kunming, Yunnan, People's Republic of China.
通讯作者:
通讯机构:[2]Department of Rheumatology and Immunology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.
推荐引用方式(GB/T 7714):
Yuan Zhongtao,Li Luqiong,Zheng Mai,et al.lncRNA TP73-AS1 Regulates miR-21/PTEN Axis to Affect Cell Proliferation in Acute Myeloid Leukemia.[J].CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS.2021,36(3):268-272.doi:10.1089/cbr.2019.3142.
APA:
Yuan Zhongtao,Li Luqiong,Zheng Mai,Xu Jian&Wang Wei.(2021).lncRNA TP73-AS1 Regulates miR-21/PTEN Axis to Affect Cell Proliferation in Acute Myeloid Leukemia..CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,36,(3)
MLA:
Yuan Zhongtao,et al."lncRNA TP73-AS1 Regulates miR-21/PTEN Axis to Affect Cell Proliferation in Acute Myeloid Leukemia.".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 36..3(2021):268-272