机构：[1]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China.昆明医科大学附属第一医院医学检验科医技科室[2]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China.[3]Yunnan Institute of Laboratory Diagnosis, Kunming, China.[4]Innovation Team of Yunnan Provincial Clinical Laboratory and Diagnosis, Kunming, China.[5]Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.[6]Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan, China.[7]Second Department of Internal Medicine, Kunming Third People's Hospital, Kunming, China.[8]Department of Gastroenterology, Kunming Children's Hospital, Kunming, China.[9]Department of Clinical Laboratory, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
This study was to screen out potential driver long non-coding RNAs (lncRNAs) in lung cancer in Xuanwei (LCXW) differently expressed mRNAs and lncRNAs were detected by gene expression microarrays in 23 paired lung adenocarcinoma and adjacent tissues. Combined bioinformatics analysis was performed to identify potential driver lncRNAs and their potential regulatory relationships. Transcriptome and clinical data in TCGA-LUAD were used as comparison and validation dataset. The comparison of LCXW and TCGA-LUAD revealed significant differences in expression of some genes, signaling pathways affected by differentially expressed genes, and the 5-year survival rate of patients. We identified 14 consistently deregulated mRNAs and 5 lncRNAs as candidate genes, which affected multiple cancer-related pathways and influenced patients' overall survival. By combined bioinformatics analysis, we further identified a potential driver lncRNA fetal-lethal non-coding developmental regulatory RNA (FENDRR) and proposed its possible regulation mechanism. The low expression of FENDRR was positively correlated with Krüppel-like factor4 (KLF4), KLF4 down-regulation may loss the activation function of cyclin-dependent kinase inhibitor 1A (CDKN1A) and cyclin-dependent kinase inhibitor 1C (CDKN1C) and the inhibition function of CyclinB1 (CCNB1), eventually cause excessive cell cycle activation and lead to lung cancer. This study revealed a potential FENDRR-KLF4-cell cycle regulation axis. These results lay an important foundation for further research on the pathogenesis of LCXW and identification of potential novel biomarkers or therapeutic targets.
第一作者机构：[1]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China.[2]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China.[3]Yunnan Institute of Laboratory Diagnosis, Kunming, China.[4]Innovation Team of Yunnan Provincial Clinical Laboratory and Diagnosis, Kunming, China.
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China.[2]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China.[3]Yunnan Institute of Laboratory Diagnosis, Kunming, China.[4]Innovation Team of Yunnan Provincial Clinical Laboratory and Diagnosis, Kunming, China.
推荐引用方式(GB/T 7714)：
Duan Yong,Li Wen-Xing,Wang Yan,et al.Integrated Analysis of lncRNAs and mRNAs Identifies a Potential Driver lncRNA FENDRR in Lung Cancer in Xuanwei, China.[J].NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL.2021,73(6):983-995.doi:10.1080/01635581.2020.1779323.
APA：
Duan Yong,Li Wen-Xing,Wang Yan,Zhao Ying,Shen Jie...&Zhang Yan-Liang.(2021).Integrated Analysis of lncRNAs and mRNAs Identifies a Potential Driver lncRNA FENDRR in Lung Cancer in Xuanwei, China..NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL,73,(6)
MLA：
Duan Yong,et al."Integrated Analysis of lncRNAs and mRNAs Identifies a Potential Driver lncRNA FENDRR in Lung Cancer in Xuanwei, China.".NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL 73..6(2021):983-995
