Condition medium of HepG-2 cells induces the transdifferentiation of human umbilical cord mesenchymal stem cells into cancerous mesenchymal stem cells.
机构：[1]Department of Gastroenterology, The Third People’s Hospital of Yunnan Province, Kunming 650011, China[2]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032,China内科科室外科科室消化内科普通外科普通外一科(胃肠与疝外科)昆明医科大学附属第一医院[3]Department of Laboratory, The Third People’s Hospital of Yunnan Province, Kunming 650000, China
出处：
ISSN：
摘要：
This study aimed to investigate the transdifferentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) into cancer-associated mesenchymal stem cells (CA-MSCs) after incubation with condition medium (CM) from liver cancer HepG-2 cells, and the biobehaviors (proliferation and migration) of these CA-MSCs were further evaluated. The supernatant of HepG-2 cells was collected and mixed with equal volume of low glucose DMEM. The resultant medium was used to treat hUCMSCs for 48 h. The expression of CA-MSCs related proteins and miR-221 was detected in cells. The supernatant of induced hUCMSCs was mixed with equal volume of high glucose DMEM, and the resultant medium was used treat HepG-2 cells for 48 h and the proliferation and migration of HepG-2 cells were evaluated. Moreover, HepG-2 cells were co-cultured with hUCMSCs and then the proliferation and migration of HepG-2 cells were assessed. After incubation with the supernatant from HepG-2 cells, hUCMSCs showed significantly elevated expression of vimentin, fibroblast activation protein (FAP) and miR-221. The supernatant of induced hUCMSCs was able to significantly increase the proliferation and migration of HepG-2 cells. Following co-culture, the proliferation and migration of HepG-2 cells increased dramatically. These findings suggest that the supernatant of HepG-2 cells is able to induce the phenotype of CA-MSCs and the supernatant of CA-MSCs may promote the proliferation and migration of HepG-2 cells. These findings provide experimental evidence for the cellular remodeling in tumor microenvironment and the safety of clinical use of hUCMSCs.
基金：
Natural Science
Foundation of Yunnan Province (2012FD095),
Key Research Project of Department of Education in Yunnan Province (2014Z125,
2015Z146), and Clinical Key Specialty Construction
Project of Yunnan Province (Yun
Health No. [2015] 18).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|3 区医学：研究与实验3 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Gastroenterology, The Third People’s Hospital of Yunnan Province, Kunming 650011, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Gastroenterology, The Third People’s Hospital of Yunnan Province, Kunming 650011, China[*1]Department of Gastroenterology, The Third People’s Hospital of Yunnan Province, Kunming 650011, China
推荐引用方式(GB/T 7714)：
Yang Juan,Miao Yinglei,Chang Yefei,et al.Condition medium of HepG-2 cells induces the transdifferentiation of human umbilical cord mesenchymal stem cells into cancerous mesenchymal stem cells.[J].American journal of translational research.2016,8(8):3429-38.
APA：
Yang Juan,Miao Yinglei,Chang Yefei,Zhang Fan,Wang Yubo&Zheng Sheng.(2016).Condition medium of HepG-2 cells induces the transdifferentiation of human umbilical cord mesenchymal stem cells into cancerous mesenchymal stem cells..American journal of translational research,8,(8)
MLA：
Yang Juan,et al."Condition medium of HepG-2 cells induces the transdifferentiation of human umbilical cord mesenchymal stem cells into cancerous mesenchymal stem cells.".American journal of translational research 8..8(2016):3429-38
