Transplantation of bone marrow stromal cells enhances infiltration and survival of CNP and Schwann cells to promote axonal sprouting following complete transection of spinal cord in adult rats.
机构:[1]Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University 295 Xichang Rd, Kunming 650032, China.外科科室神经外科神经外一科(神经外科)昆明医科大学附属第一医院[2]Department of Ophthalmology, Yunnan NO.2 Provincial People's Hospital 176 Qingnian Rd, Kunming 650021, China.
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摘要:
This study aimed to investigate the roles of bone marrow stromal cells (BMSCs) in promoting axonal regeneration after complete transection of spinal cord in adult rats. Transplantation was done 9 days after injury. Only a few BMSCs were detected at the injury site 8 weeks after transplantation, yet there was robust growth of axons. The scarcity of surviving BMSCs may attribute to the adverse conditions in their ambient environment. In this connection, the immediate accumulation of a large number of macrophages/reactive microglia following BMSCs transplantation and subsequent cavitation of tissues may be detrimental to their survival. An unexpected finding following BMSCs transplantation was the marked increase in the nestin, GFAP, NF200, olig 3 and CNP positive cells at the injury site. Immunoelectron microscopy showed CNP cells were oval or fibroblast-like and had multiple perineurial-like compartments with long extending filopodia. The spatial relationship between regenerating axons and CNP-positive cells was also confirmed by double immunofluorescence staining. Our results suggest that transplantation of BMSCs elicits the influx and survival of local cells including CNP positive cells and Schwann cells into injury site, which provide structural support for the axon regeneration and remyelination after spinal cord injury.
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外文
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出版当年[2015]版:
大类|3 区医学
小类|3 区医学:研究与实验3 区肿瘤学
最新[2023]版:
大类|4 区医学
小类|4 区医学:研究与实验4 区肿瘤学
第一作者:
第一作者机构:[1]Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University 295 Xichang Rd, Kunming 650032, China.
推荐引用方式(GB/T 7714):
Ding Peng,Yang Zhiyong,Wang Weimin,et al.Transplantation of bone marrow stromal cells enhances infiltration and survival of CNP and Schwann cells to promote axonal sprouting following complete transection of spinal cord in adult rats.[J].American journal of translational research.2014,6(3):224-35.
APA:
Ding Peng,Yang Zhiyong,Wang Weimin,Wang Jinkun&Xue Liping.(2014).Transplantation of bone marrow stromal cells enhances infiltration and survival of CNP and Schwann cells to promote axonal sprouting following complete transection of spinal cord in adult rats..American journal of translational research,6,(3)
MLA:
Ding Peng,et al."Transplantation of bone marrow stromal cells enhances infiltration and survival of CNP and Schwann cells to promote axonal sprouting following complete transection of spinal cord in adult rats.".American journal of translational research 6..3(2014):224-35