Study on the Expression Spectrum of miRNAs in Peripheral Blood from Patients and the Value of miR-548h-5p in the Diagnosis of Chronic Obstructive Pulmonary Disease
To seek novel miRNAs for the diagnosis of Chronic Obstructive Pulmonary Disease (COPD), this study analyzed the differential expression of miRNA from blood samples of COPD patients and healthy smokers (n = 3) by human microarray analysis. Then, some miRNAs were chosen for qRT-PCR validation. A total of 158 miRNAs revealed by microarray analysis have been differentially expressed between the two groups, 33 miRNAs identified to be up-regulated, whereas 125 miRNAs have been down-regulated in COPD. qRT-PCR showed the miR-548h-5p was decreased in the COPD patients compared with healthy smokers (p = 0.04). The sensitivity and specificity of miR-548h-5p to the diagnosis of COPD were 84.6% and 81.8%, respectively. In conclusion, it is recommend that miR-548h-5p as an approach to the diagnosis of COPD.
基金：
Health and Family Planning Commission of Yunnan Province, China (Foundation of Yunnan Health Science and Technology Project) [2017NS046]; Health and Family Planning Commission of Yunnan Province, China (National Natural Science Foundation of China)National Natural Science Foundation of China (NSFC) [81860013]
第一作者机构：[1]Kunming Med Univ, Dept Resp Crit Care Med, Affiliated Hosp 1, Kunming 650032, Yunnan, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Li Chun-Tao,Zhang Jian-Qing,Dai Lu-Ming,et al.Study on the Expression Spectrum of miRNAs in Peripheral Blood from Patients and the Value of miR-548h-5p in the Diagnosis of Chronic Obstructive Pulmonary Disease[J].NANOSCIENCE AND NANOTECHNOLOGY LETTERS.2020,12(11):1323-1328.doi:10.1166/nnl.2020.3246.
APA：
Li, Chun-Tao,Zhang, Jian-Qing,Dai, Lu-Ming,Zhang, Jia-Qiang,Fang, Li-Zhou&Feng, Jia-Gang.(2020).Study on the Expression Spectrum of miRNAs in Peripheral Blood from Patients and the Value of miR-548h-5p in the Diagnosis of Chronic Obstructive Pulmonary Disease.NANOSCIENCE AND NANOTECHNOLOGY LETTERS,12,(11)
MLA：
Li, Chun-Tao,et al."Study on the Expression Spectrum of miRNAs in Peripheral Blood from Patients and the Value of miR-548h-5p in the Diagnosis of Chronic Obstructive Pulmonary Disease".NANOSCIENCE AND NANOTECHNOLOGY LETTERS 12..11(2020):1323-1328
