机构：[1]Department of Clinical Laboratory, the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming, China[2]Department of Clinical Laboratory, the First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Laboratory Diagnosis, Yunnan Key Laboratory of Laboratory Medicine, Kunming , China昆明医科大学附属第一医院医技科室医学检验科
Background: Carbapenem-resistant Enterobacteriaceae (CRE) has become a public health threat due to resistance to multiple antibiotics. The production of fi -lactamase is the most important resistance mechanism of Enterobacteriaceae. Although isolates producing KPC-2 or NDM-1 enzymes have been reported widely, isolates co-producing KPC-2, NDM-1, TEM-1, TEM-95, SHV-66, and other fi-lactamases have rarely been detected in the same strain, especially in Enterobacter cloacae. Objectives: In this study, we identified and sequenced the genome of carbapenem-resistant E. cloacae ECL189 to in-depth analyze the resistance and transmission mechanisms of E. cloacae. Methods: We investigated the antimicrobial susceptibility of ECL189 by a VITEK 2 system, E-test gradient strips, and K-B method. Whole-genomesequencingwasused by the PacBio RS II platformandIllumina HiSeq4000platform. Antimicrobial resistance genes, virulence genes, non-coding RNA, and repeat sequences were predicted by biological information databases. A PCR was used to further confirm that the blaKPC-2, blaNDM-1, blaTEM-1, blaTEM-95, and blaSHV-66 genes existed in ECL189. A conjugation experiment was performed to determine the transferability of resistance. Molecular typing of ECL189 was done by multilocus sequence typing (MLST). Results: Enterobacter cloacae ECL189 was resistant to 21 out of 23 tested antibiotics, but its transconjugant was resistant to eight out of 18 tested antibiotics. The genome of ECL189 consisted of a 5,026,406 bp chromosome and four circular plasmids. In total, 26 resistance genes and 58 resistance proteins were identified. In addition, 77 determinants associated with bacterial virulence were identified. A large number of resistance and virulence genes were located in the plasmids. The results of whole-genome sequencing were consistent with the fi-lactamase genes. The MLST analysis revealed that this strain belonged to ST74. Conclusions: This study further revealed the resistance, virulence, and transmission mechanisms of carbapenem-resistant E. cloacae. Resistance and virulence genes spread in bacteria by the horizontal transfer of plasmids, which should attract more attention in relevant departments.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81960382]; Yunnan Provincial Science and Technology DepartmentTechnology Planning Project [2018FA043]; Yunnan Province Health and Family Planning Commission Medical Discipline Leader Training Program [D-2017023]
第一作者机构：[1]Department of Clinical Laboratory, the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming, China
通讯作者：
通讯机构：[2]Department of Clinical Laboratory, the First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Laboratory Diagnosis, Yunnan Key Laboratory of Laboratory Medicine, Kunming , China[*1]Department of Clinical Laboratory, the First Affiliated Hospital of Kunming Medical University, Yunnan Key Laboratory of Laboratory Medicine, Yunnan Institute of Laboratory Diagnosis, No. 295 Xichang Rd,Wuhua District, Kunming, China
推荐引用方式(GB/T 7714)：
Du Na,Liu Shumin,Yao Jing,et al.Genomic Characterization of Multidrug-resistant Carbapenemase-Producing Enterobacter cloacae ECL189, Co-producing KPC-2, NDM-1, TEM-1, TEM-95, and SHV-66[J].JUNDISHAPUR JOURNAL OF MICROBIOLOGY.2020,13(11):doi:10.5812/jjm.105761.
APA：
Du, Na,Liu, Shumin,Yao, Jing,Yang, Kai,Lin, Yun...&Du, Yan.(2020).Genomic Characterization of Multidrug-resistant Carbapenemase-Producing Enterobacter cloacae ECL189, Co-producing KPC-2, NDM-1, TEM-1, TEM-95, and SHV-66.JUNDISHAPUR JOURNAL OF MICROBIOLOGY,13,(11)
MLA：
Du, Na,et al."Genomic Characterization of Multidrug-resistant Carbapenemase-Producing Enterobacter cloacae ECL189, Co-producing KPC-2, NDM-1, TEM-1, TEM-95, and SHV-66".JUNDISHAPUR JOURNAL OF MICROBIOLOGY 13..11(2020)
