机构：[1]NHC Key Laboratory of Drug Addiction Medicine, Kunming Medical University, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.昆明医科大学附属第一医院国家卫生健康委毒品依赖和戒治重点实验室国家级重点实验室[2]Centre for Experimental Studies and Research, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.昆明医科大学附属第一医院[3]Medical School, Kunming University of Science and Technology, Kunming 650032, Yunnan, China.[4]Yunnan Institute of Digestive Disease, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.昆明医科大学附属第一医院省级研究所云南省消化疾病研究所[5]School of Public Health, Kunming Medical University, Kunming 650032, Yunnan, China.[6]Yunnan University, Kunming 650032, Yunnan, China.
Heroin addiction and withdrawal have been associated with an increased risk for infectious diseases and psychological complications. However, the changes of metabolites in heroin addicts during withdrawal remain largely unknown.A total of 50 participants including 20 heroin addicts with acute abstinence stage, 15 with protracted abstinence stage and 15 healthy controls, were recruited. We performed metabolic profiling of plasma samples based on ultraperformance liquid chromatography coupled to tandem mass spectrometry to explore the potential biomarkers and mechanisms of heroin withdrawal.Among the metabolites analyzed, omega-6 polyunsaturated fatty acids (linoleic acid, dihomo-gamma-linolenic acid, arachidonic acid, n-6 docosapentaenoic acid), omega-3 polyunsaturated fatty acids (docosahexaenoic acid, docosapentaenoic acid), aromatic amino acids (phenylalanine, tyrosine, tryptophan), and intermediates of the tricarboxylic acid cycle (oxoglutaric acid, isocitric acid) were significantly reduced during acute heroin withdrawal. Although majority of the metabolite changes could recover after months of withdrawal, the levels of alpha-aminobutyric acid, alloisoleucine, ketoleucine, and oxalic acid do not recover.In conclusion, the plasma metabolites undergo tremendous changes during heroin withdrawal. Through metabolomic analysis, we have identified links between a framework of metabolic perturbations and withdrawal stages in heroin addicts.
基金：
the National Natural Science Foundation of China (Grant Nos. 81860100, 81860306, and 81870458), Yunnan Province Clinical Research Center (2019ZF012), Yunling Scholar (Grant No. YLXL20170002), and Scientific Research Fund of Yunnan Provincial Department of Education (2021J0234), Digitalization, Development and Application of Biotic Resource (202002AA100007), Science and Technology Department of Yunnan Province (Grant Nos. 2017FE468(-142), 2018DH006, 2018NS0086, 2019FE001(-218), 202001AV070010), NHC Key Laboratory of Drug Addiction Medicine (2020DAMARA-004).
第一作者机构：[1]NHC Key Laboratory of Drug Addiction Medicine, Kunming Medical University, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.[2]Centre for Experimental Studies and Research, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.
共同第一作者：
通讯作者：
通讯机构：[1]NHC Key Laboratory of Drug Addiction Medicine, Kunming Medical University, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.[2]Centre for Experimental Studies and Research, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.[6]Yunnan University, Kunming 650032, Yunnan, China.
推荐引用方式(GB/T 7714)：
Zhou Yong,Xie Zhenrong,Zhang Zunyue,et al.Plasma metabolites changes in male heroin addicts during acute and protracted withdrawal.[J].AGING-US.2021,13(14):18669-18688.doi:10.18632/aging.203311.
APA：
Zhou Yong,Xie Zhenrong,Zhang Zunyue,Yang Jiqing,Chen Minghui...&Wang Kunhua.(2021).Plasma metabolites changes in male heroin addicts during acute and protracted withdrawal..AGING-US,13,(14)
MLA：
Zhou Yong,et al."Plasma metabolites changes in male heroin addicts during acute and protracted withdrawal.".AGING-US 13..14(2021):18669-18688
