机构：[1]Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China医技科室医学影像中心昆明医科大学附属第一医院[2]Department of Obstetrics and Gynecology, Luoyang CITIC Central Hospital, Luoyang 471003, China
The aim of this study was to clarify the role of lncRNA metastasis-associated lung adenocarcinoma tran-script 1 (MALAT1) in proliferation, migration, and invasion of malignant pleural mesothelioma (MPM) cells. The quan-titative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the expression of MALAT1 in MPM cell lines. The effects of MALAT1 and miR-141-3p on the proliferation, migration, and invasion of MPM cells were studied through a series of in vitro cellular experi-ments. The flow cytometry was utilized to detect the cell apoptosis. The dual-luciferase reporter assay was employed to explore the binding relationship among MALAT1, miR-141-3p, and YES-associated protein 1 (YAP1). MALAT1 was overexpressed in MPM cell lines, while its knockdown significantly inhibited the cell proliferation, migration, and invasion, and increased the number of MPM cells in the G0/G1 phase. In addition, MALAT1 could directly bind to miR-141-3p and inhibit its expression. YAP1 has been iden-tified as a downstream target of miR-141-3p, and its expres-sion level was inhibited by miR-141-3p. MALAT1 can be used as a competitive endogenous RNA (ceRNA) to regulate the YAP1-Hippo signaling pathway through miR-141-3p, pro -mote the proliferation, migration, and invasion of MPM cells, and provide a new target for the therapy of MPM.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [8196070113]
第一作者机构：[1]Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
通讯作者：
通讯机构：[1]Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China[*1]Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming 650032, China
推荐引用方式(GB/T 7714)：
Wang Pei,Bai Cuiwei,Shen Shasha,et al.MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p[J].OPEN MEDICINE.2021,16(1):1653-1667.doi:10.1515/med-2021-0383.
