机构：[1]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China昆明医科大学附属第一医院[2]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China[3]Yunnan Province Clinical Research Center for Laboratory Medicine, Kunming, China
Carbapenem-resistant P. aeruginosa (CRPA) is particularly worrisome because of its resistance against multiple antimicrobial agents which reduces treatment options. The efflux pump decreases antibiotic abundance, and biofilm impairs the penetration of antibiotics. The aim of the present study was to evaluate the role and relationship of efflux pump and biofilm formation in CRPA isolates obtained from different clinical samples.A total of 110 different clinical samples were collected from three tertiary medical hospitals. The samples were subjected to isolation and identification by standard operating procedures. Species level were identified using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system. Antibiotic susceptibility testing was done by broth microdilution method. Crystal violet (CV) staining for observing the biofilm forming ability and amplification of efflux pump mexA gene were also performed on clinical CRPA isolates. Three efflux pump MexAB-OprM regulatory genes were analyzed using sequencing methods. The expression of mexA gene both in biofilm and planktonic bacteria was observed by Quantitative real-time PCR (qRT-PCR).The results showed that 110 samples were CRPA and among them 83 (75.5%) were MDR isolates. The CV staining showed 105 (95.5%) isolates as biofilm producers while 78 (74.3%) MDR isolates showed biofilm formation. mexA hyperexpression was detected in 27 (24.5%) CRPA isolates while 26 (96.3%) in biofilm forming isolates and 96.3% (26/27) in MDR P. aeruginosa. Multiple mutations in nalC, nalD, and mexR genes were detected. The distinct difference confirmed that the expression of mexA gene in P. aeruginosa biofilm producer was significantly higher than that of planktonic bacteria in vitro, and the efflux pump inhibitor PAβN significantly inhibited biofilms in CRPA isolated from clinical samples.The biofilm and efflux pumps might be two intertwined processes involved in CRPA isolates. Their synergistic effect magnified the drug resistance characteristics of P. aeruginosa.
基金：
This work was supported by grants from Special Foundation
for National Science and Technology Basic Research
Program of China (2019FY101200, 2019FY101209), Yunnan
health training project of high-level talents (Grant No:
H-2018075) and Yunnan High-level Personnel training support
plan (Grant No: YNWR-QNBJ-2020-261).
第一作者机构：[1]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China[2]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China[3]Yunnan Province Clinical Research Center for Laboratory Medicine, Kunming, China
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China[2]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China[3]Yunnan Province Clinical Research Center for Laboratory Medicine, Kunming, China
推荐引用方式(GB/T 7714)：
Li X-F,Shi H-Q,Liang Y,et al.Interaction of biofilm and efflux pump in clinical isolates of carbapenem resistant P. aeruginosa.[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2022,26(5):1729-1737.doi:10.26355/eurrev_202203_28242.
APA：
Li X-F,Shi H-Q,Liang Y,Li J,Jiang B&Song G-B.(2022).Interaction of biofilm and efflux pump in clinical isolates of carbapenem resistant P. aeruginosa..EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,26,(5)
MLA：
Li X-F,et al."Interaction of biofilm and efflux pump in clinical isolates of carbapenem resistant P. aeruginosa.".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 26..5(2022):1729-1737
