机构：[1]National Resource Center for Non-Human Primates, Kunming Primate Research Center, and National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China[2]Institutes of Physical Science and Information Technology，Anhui University, Hefei, China[3]First Affiliation Hospital of Kunming Medical University, Kunming, China[4]Department of Pharmacology, School of Medicine, Southeast University, Nanjing, Jiangsu, China[5]School of Chinese Materia Medica, Yunnan University of Chinese Medicine. Kunming, Yunnan, China[6]Department of Neurosurgery, Second Affiliated Hospital of Nanchang University, Nanchang, China[7]Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, State Key Laboratory of Neuroscience, Shanghai, China
Background: Ischemic stroke leads to a long-term disability in humans and no efficient clinical therapy exists to date. The middle cerebral artery occlusion (MCAO) model in non-human primates has shown to be of value for translational stroke research. New method In the current study, a photothrombotic (PT) stroke model was established in rhesus monkeys with either a proximal or distal segment of middle cerebral artery (MCA) thrombosis. This study is the first that compares the two approaches of PT stroke in monkeys using behavioral and physiological measurements and MRI scans. Results: The experiment found that infarct occurred in the MCA target regions, with all monkeys having impaired behavior reflected by deficits in neurologic function, and motor and cognition in object retrieval detour (ORD) task. The monkeys with distal MCA thrombosis developed with sequential photo-irritations of the Sylvian fissure zone, adjacent central anterior gyrus and central posterior gyrus, had similar impairments with respect to behavior and showed a tendency of a small edema volume with proximal MCA thrombosis at days 4 and 7 post PT stroke. Comparison with existing methods: The distal MCA thrombosis developed with sequential photo-irritations might provide a consistent and well-tolerated focal ischemia in rhesus monkeys, compared with other PT stroke models which usually were singly conducted on the animal's motor cortex and had a temporal effect. Conclusions: The sequentially photo-irritated PT stroke model is a promising ischemic stroke model in rhesus monkey for studying human stroke pathology and physiology and for new therapies development.
基金：
The study was supported by National Key Research and Development
Program of China (2018YFC1313803), Yunnan Key Program of Science
and Technology (2017FA042, 2018IA095), National Science Foundation
of China (31571109, 31771194, 81860252, 81703497).
第一作者机构：[1]National Resource Center for Non-Human Primates, Kunming Primate Research Center, and National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China[2]Institutes of Physical Science and Information Technology，Anhui University, Hefei, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhiting Zhang,Shuguo Wang,Lingli Du,et al.A pilot behavioural and neuroimaging investigation on photothrombotic stroke models in rhesus monkeys[J].JOURNAL OF NEUROSCIENCE METHODS.2021,362:doi:10.1016/j.jneumeth.2021.109291.
APA：
Zhiting Zhang,Shuguo Wang,Lingli Du,Ling Xu,Yu Lin...&Jianhong Wang.(2021).A pilot behavioural and neuroimaging investigation on photothrombotic stroke models in rhesus monkeys.JOURNAL OF NEUROSCIENCE METHODS,362,
MLA：
Zhiting Zhang,et al."A pilot behavioural and neuroimaging investigation on photothrombotic stroke models in rhesus monkeys".JOURNAL OF NEUROSCIENCE METHODS 362.(2021)
