机构：[1]Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China医学检验科医技科室[2]Yunnan Institute of Experimental Diagnosis, Kunming 650032, Yunnan, China医学检验科医技科室普通外三科(血管外科)普通外科外科科室[3]Yunnan Key Laboratory of Laboratory Medicine, Kunming 650032, Yunnan, China医学检验科医技科室普通外三科(血管外科)普通外科外科科室[4]Yunnan Engineering Technology Center of Diagnosis and Treatment of Digestive Diseases, Kunming 650032, Yunnan, China医学检验科医技科室普通外三科(血管外科)普通外科外科科室[5]Department of Vascular Surgery, The First Afliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming 650032, Yunnan, China医学检验科医技科室普通外三科(血管外科)普通外科外科科室
To explore the role of the miRNA-1297/phospholipase C beta 1 (PLC beta 1) axis in intestinal barrier injury. Abnormally expressed miR-1297 and its target gene PLC beta 1 as well as their transcriptome sequencing were confirmed by bioinformatics analysis. Next, the intestinal barrier injury was induced by lipopolysaccharide (LPS) in the CCCHIE-2 cells. Subsequently, the impacts of miR-1297 and PLC beta 1 on the transcriptome were estimated. QRT-PCR and Western blotting were conducted to detect the relative mRNA and protein expressions, respectively. The cell viability and permeability were analyzed by MTT assay and fluorescent yellow detection. miR-1297 was significantly upregulated in patients with human immunodeficiency virus/acquired immunodeficiency syndrome and targeted PLC beta 1. Moreover, overexpressed PLC beta 1 was mainly enriched in the transforming growth factor-beta signaling pathway, while the knockdown of miR-1297 was focused on the arginine biosynthesis pathway. The overexpression of miR-1297 could reduce the PLC beta 1 expression and inhibit the viability of CCCHIE-2 cells injured by LPS, while the effect of the downregulation of miR-1297 was on the opposite. Western blotting and cell fluorescence localization experiments revealed that the inhibition of miR-1297 increased the expressions of PLC beta 1 and ZO-1. In addition, the upregulation of miR-1297 strengthened the permeability in cells injured by LPS, as did the knockdown of PLC beta 1. miR-1297 could restrain the repair of intestinal barrier injury via negatively regulating PLC beta 1 and its tight junction downstream protein ZO-1 in CCC-HIE-2 cells injured by LPS, which indicated that PLC beta 1 and miR-1297 might be important targets for the repair of intestinal barrier injury.
基金：
Basic Research Plan of Yunnan Province (Joint Project of Kunming Medical College) (No. 2019FE001 (-221))(No. 2019FE001 (-221))
第一作者机构：[1]Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China[2]Yunnan Institute of Experimental Diagnosis, Kunming 650032, Yunnan, China[3]Yunnan Key Laboratory of Laboratory Medicine, Kunming 650032, Yunnan, China
通讯作者：
通讯机构：[4]Yunnan Engineering Technology Center of Diagnosis and Treatment of Digestive Diseases, Kunming 650032, Yunnan, China
推荐引用方式(GB/T 7714)：
Yuxia Bao,Jianyuan Gao,Bin Yang,et al.MicroRNA‑1297 participates in the repair of intestinal barrier injury in patients with HIV/AIDS via negative regulation of PLCβ1[J].Molecular and Cellular Biochemistry.2022,doi:10.1007/s11010-022-04426-z.
APA：
Yuxia Bao,Jianyuan Gao,Bin Yang,Fengrong Chen,Zunyue Zhang&Jianyuan Gao.(2022).MicroRNA‑1297 participates in the repair of intestinal barrier injury in patients with HIV/AIDS via negative regulation of PLCβ1.Molecular and Cellular Biochemistry,,
MLA：
Yuxia Bao,et al."MicroRNA‑1297 participates in the repair of intestinal barrier injury in patients with HIV/AIDS via negative regulation of PLCβ1".Molecular and Cellular Biochemistry .(2022)
