Background Myocardial ischemia-reperfusion injury (MI/RI) is a primary cause of cardiomyocyte death in various cardiovascular diseases. MicroRNAs (miRNAs) play significant roles in MI/RI.Objective This study aims to elucidate the function and mechanism of miR-379-3p in this context.Methods The Cell Counting Kit 8 (CCK-8) and flow cytometry were utilized to assess the proliferation and apoptosis of cardiomyocytes. The expression levels of cardiomyocyte injury-related proteins, including LDHA, cTnT, CK-MB, and cTnI, were measured using enzyme-linked immunoassay (ELISA) kits. The targeted regulatory relationship between miR-379-3p and dual-specificity protein phosphatase 1 (DUSP1) was investigated through dual luciferase reporter gene assays. Additionally, the expression levels of miR-379-3p/DUSP1 and mitogen-activated protein kinases (MAPKs) signaling pathway in MI/RI were evaluated using Western blotting.Results The expression of miR-379-3p was significantly elevated in MI/RI, and the inhibition of miR-379-3p notably alleviated MI/RI. The specific binding of miR-379-3p to the 3'UTR of DUSP1 mRNA was confirmed by dual luciferase reporter gene assays. Furthermore, miR-379-3p exacerbated cardiomyocyte injury by negatively regulating DUSP1. Ultimately, miR-379-3p activated the MAPK/JNK/p38 pathway by downregulating DUSP1 expression, thereby promoting MI/RI.Conclusions miR-379-3p activates the MAPK/JNK/p38 pathway through the negative regulation of DUSP1 expression to promote MI/RI.
基金：
Joint Special Fund for Applied Basic Research of Kunming Medical University [2019FE001[-045]]; Yunnan Health Training Project of High-Level Talents [H-2019028]
基金编号：2019FE001[-045]H-2019028
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2026]版：
无
最新[2025]版：
大类|4 区医学
小类|4 区血液学4 区外周血管病
第一作者：
第一作者机构：[1]Kunming Med Univ, Dept Anesthesiol, Affiliated Hosp 1, Kunming, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Wei,Bai Xiangfeng,Tang Yao,et al.miR-379-3p activates the MAPK/JNK/p38 pathway by negatively targeting DUSP1 expression to promote myocardial ischemia-reperfusion injury[J].CLINICAL HEMORHEOLOGY AND MICROCIRCULATION.2025,doi:10.1177/13860291251348806.
APA：
Yang, Wei,Bai, Xiangfeng,Tang, Yao,Ren, Ziting&Chen, Wendong.(2025).miR-379-3p activates the MAPK/JNK/p38 pathway by negatively targeting DUSP1 expression to promote myocardial ischemia-reperfusion injury.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,,
MLA：
Yang, Wei,et al."miR-379-3p activates the MAPK/JNK/p38 pathway by negatively targeting DUSP1 expression to promote myocardial ischemia-reperfusion injury".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION .(2025)
