Objective Draw upon research into the serum concentration, mRNA expression, and DNA methylation of TNF-like weak inducer of apoptosis (TWEAK) in the peripheral blood of systemic lupus erythematosus patients and healthy controls in an attempt to investigate the epigenetics associated with TWEAK in the pathogenesis of systemic lupus erythematosus (SLE). Methods A total of 178 SLE patients (SLE group) and 131 sex-age matched healthy controls (HC group) were recruited. Enzyme-linked immunosorbent assays (ELISA) was used to detect serum protein concentration of TWEAK. TWEAK mRNA expression was analyzed by Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Methylation levels of the promotor of TWEAK were measured using quantitative DNA methylation analysis on the MassARRAY spectrometry. Results Serum TWEAK concentrations were not statistically significant in SLE patients and HCs. Nevertheless, serum TWEAK concentrations were significantly lower in patients with renal involvement when compared to those without it. Serum TWEAK concentrations were reduced in clinically active patients (SLEDAI >= 10) compared with clinically stable patients (SLEDAI < 10). It was also significantly associated with SLEDAI. Compared with the HC group, the TWEAK mRNA expression in the SLE group was significantly lower. The global DNA methylation levels of TWEAK in the SLE group were observed to be significantly higher than the HC group. SLE patients with renal involvement, and the clinically active patients had higher TWEAK global methylation as well as exhibited variation in certain CpG island methylation. Furthermore, TWEAK methylation negatively correlated with TWEAK mRNA expression. Conclusion This study suggests that TWEAK DNA methylation is a valuable as a focus for epigenetic studies because of it potentially influencing TWEAK gene expression in SLE patients. Aberrant DNA methylation of TWEAK may be involved in the initiation and development of SLE.
基金:
National Natural Science Foundation of China [81760296, 32270947, 82060259, 81460256]; Yunnan Province High-level health technical talents [L-2019004, L-2019011]; Yunnan Province Special Project for Famous Medical Talents of the "Ten Thousand Talents Program" [YNWRMY-2018-040, YNWR-MY-2018-041]; Funding of Yunnan Provincial Health Science and Technology Plan [2017NS051, 2017NS052, 2018NS0133]; Funding of Ministry of Science and Technology of Yunnan Province [2018ZF016]; Yunnan Province Clinical Research Center for Skin Immune Diseases [2019ZF012]; Yunnan Province Clinical Center for Skin Immune Diseases [ZX2019-03-02]
第一作者机构:[1]Kunming Med Univ, Affiliated Hosp 1, Dept Rheumatol & Immunol, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China
共同第一作者:
通讯作者:
通讯机构:[1]Kunming Med Univ, Affiliated Hosp 1, Dept Rheumatol & Immunol, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China[*1]Department of Rheumatology and Immunology, First Affiliated Hospital of Kunming Medical University, No.295 Xichang Road, Kunming, Yunnan, 650032, China
推荐引用方式(GB/T 7714):
Liao Li,Li Shu,Upreti Bibhuti,et al.Status of TWEAK DNA methylation and mRNA expression in systemic lupus erythematosus[J].LUPUS.2023,32(2):171-179.doi:10.1177/09612033221141261.
APA:
Liao, Li,Li, Shu,Upreti, Bibhuti,Wang, Xiangyu,Yang, Yifan...&Xu, Jian.(2023).Status of TWEAK DNA methylation and mRNA expression in systemic lupus erythematosus.LUPUS,32,(2)
MLA:
Liao, Li,et al."Status of TWEAK DNA methylation and mRNA expression in systemic lupus erythematosus".LUPUS 32..2(2023):171-179