机构:[1]Department of Rheumatology and Immunology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy ofMedical Sciences, Beijing, PR China[2]Department of Rheumatology and Immunology, the First Affiliated Hospital of Kunming MedicalUniversity, Kunming, Yunnan, PR China内科科室风湿免疫科昆明医科大学附属第一医院[3]Department of Psychiatry, the First Affiliated Hospital of Kunming Medical University, Kunming,Yunnan, PR China内科科室精神科昆明医科大学附属第一医院[4]State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences,Kunming, Yunnan, PR China[5]Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, ChineseAcademy of Sciences, Kunming, Yunnan, PR China
A higher prevalence of depression in systemic lupus erythematosus (SLE) patients has been reported, though the mechanism underlying this phenomenon remains unclear. The present study was conducted to explore whether the polymorphism and methylation status of the serotonin transporter gene (5HTT) promoter region (PR-5HTT) contribute to depression in SLE patients from both genetic and epigenetic perspectives. In this study, 96 SLE patients and 96 healthy controls (HCs) were recruited. Depression levels of all subjects were evaluated using the Hamilton Depression Rating Scale (HDRS). The serotonin transporter-linked polymorphism (5HTTLPR) and the DNA methylation status of PR-5HTT were detected in peripheral lymphocytes of SLE patients and HCs. The differences in 5HTTLPR and DNA methylation of PR-5HTT between SLEs and HCs were compared. In SLE patients, the frequencies of short allele (S) and SS genotype of 5HTTLPR were higher in depressive SLE (SLE-D) patients than in non-depressive SLE (SLE-ND) patients. The mean HDRS score of SS homozygote patients was higher than that of patients with SL/LL genotypes. Conversely, PR-5HTT was hypomethylated in HCs as well as SLE patients. There was no difference in the methylation status between HCs and SLEs. Thus, the functional expression of PR-5HTT may be primarily regulated by gene polymorphism and not by DNA methylation. The risk allele of 5HTTLPR appears to be a major contributor to depression in SLE patients.
基金:
Supporting Program of the "Eleventh Five-year Plan" for Science and Technology Research of China [2008BAI59B02, 2008BAI59B03]; National High Technology Research and Development Program of China (863 Program)National High Technology Research and Development Program of China [2012AA02A513]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81101005, 81160379, 81160171]; Yunnan Provincial Science and Technology Department; Kunming Medical University [2011FB167]; Yunnan Provincial Health Science and Technology Plan [2010NS016, 2011WS008]; Yunnan Provincial Public Health for Academic Leadership [D-201218, D-201239]
第一作者机构:[1]Department of Rheumatology and Immunology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy ofMedical Sciences, Beijing, PR China[2]Department of Rheumatology and Immunology, the First Affiliated Hospital of Kunming MedicalUniversity, Kunming, Yunnan, PR China
共同第一作者:
通讯作者:
通讯机构:[*1]Department of Rheumatology and Immunology, Peking Union Medical College Hospital (West), Peking Union Medical College, Chinese Academy of Medical Sciences, No. 41 Damucang Hutong, Xicheng District, Beijing, China 100032.
推荐引用方式(GB/T 7714):
J Xu,YQ Cheng,B Chen,et al.Depression in systemic lupus erythematosus patients is associated with link-polymorphism but not methylation status of the 5HTT promoter region[J].LUPUS.2013,22(10):1001-1010.doi:10.1177/0961203313498793.
APA:
J Xu,YQ Cheng,B Chen,R Bai,S Li...&XF Zeng.(2013).Depression in systemic lupus erythematosus patients is associated with link-polymorphism but not methylation status of the 5HTT promoter region.LUPUS,22,(10)
MLA:
J Xu,et al."Depression in systemic lupus erythematosus patients is associated with link-polymorphism but not methylation status of the 5HTT promoter region".LUPUS 22..10(2013):1001-1010