机构：[1]Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.[2]Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, China.[3]Department of Clinical Laboratory,First Affiliated Hospital of Kunming Medical University,Kunming,China.昆明医科大学附属第一医院医技科室医学检验科[4]Department of Clinical Laboratory, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai, China.
OXA-232 carbapenemase is becoming a threat in China due to its high prevalence, mortality, and limited treatment options. However, little information is available on the impact of OXA-232-producing Klebsiella pneumoniae in China. This study aims to characterize the clonal relationships, the genetic mechanisms of resistance, and the virulence of OXA-232-producing K. pneumoniae isolates in China. We collected 81 OXA-232-producing K. pneumoniae clinical isolates from 2017 to 2021. Antimicrobial susceptibility testing was performed using the broth microdilution method. Capsular types, multilocus sequence types, virulence genes, antimicrobial resistance (AMR) determinants, plasmid replicon types, and single-nucleotide polymorphism (SNP) phylogeny were inferred from whole-genome sequences. OXA-232-producing K. pneumoniae strains were resistant to most antimicrobial agents. These isolates showed partial differences in susceptibility to carbapenems: all strains were resistant to ertapenem, while the resistance rates to imipenem and meropenem were 67.9% and 97.5%, respectively. Sequencing and capsular diversity analysis of the 81 K. pneumoniae isolates revealed 3 sequence types (ST15, ST231, and one novel ST [ST-V]), 2 K-locus types (KL112 and KL51), and 2 O-locus types (O2V1 and O2V2). The predominant plasmid replicon types associated with the OXA-232 and rmtF genes were ColKP3 (100%) and IncFIB-like (100%). Our study summarized the genetic characteristics of OXA-232-producing K. pneumoniae circulating in China. The results demonstrate the practical applicability of genomic surveillance and its utility in providing methods to prevent transmission. It alerts us to the urgent need for longitudinal surveillance of these transmissible lineages. IMPORTANCE In recent years, the detection rate of carbapenem-resistant K. pneumoniae has increased and represents a major threat to clinical anti-infective therapy. Compared with KPC-type carbapenemases and NDM-type metallo-β-lactamases, OXA-48 family carbapenemases are another important resistance mechanism mediating bacterial resistance to carbapenems. In this study, we investigated the molecular characteristics of OXA-232 carbapenemase-producing K. pneumoniae isolated from several hospitals to clarify the epidemiological dissemination characteristics of such drug-resistant strains in China.
基金：
National Natural Science Foundation of China
(grants 81871690, 82172311, and 32141002) and the China Antimicrobial Surveillance
Network (grant 2020QD049).
第一作者机构：[1]Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.[2]Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, China.
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.[2]Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, China.
推荐引用方式(GB/T 7714)：
Shen Siquan,Han Renru,Yin Dandan,et al.A Nationwide Genomic Study of Clinical Klebsiella pneumoniae Carrying blaOXA-232 and rmtF in China[J].MICROBIOLOGY SPECTRUM.2023,11(3):doi:10.1128/spectrum.03863-22.
APA：
Shen Siquan,Han Renru,Yin Dandan,Jiang Bo,Ding Li...&Hu Fupin.(2023).A Nationwide Genomic Study of Clinical Klebsiella pneumoniae Carrying blaOXA-232 and rmtF in China.MICROBIOLOGY SPECTRUM,11,(3)
MLA：
Shen Siquan,et al."A Nationwide Genomic Study of Clinical Klebsiella pneumoniae Carrying blaOXA-232 and rmtF in China".MICROBIOLOGY SPECTRUM 11..3(2023)
