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Transcriptome-wide profile of 1a,25 dihydroxyvitamin D-3 in HTR-8/SVneo cells

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收录情况： ◇ SCIE

作者：
Luo, Jiahuan[1,2] ; Tang, Li[1,2,*1] ; Zhang, Ruopeng[3,4,*2] ; Lv, Mengxin[3] ; You, Dingyun[5] ; Yuan, Qian[3,6,*2] ;
机构： [1]Department of Reproductive Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China [2]Graduate School, Kunming Medical University, Kunming, Yunnan, China [3]Medical Genetics and Prenatal Diagnosis Center, Kunming Maternal and Child Health Hospital, Kunming, Yunnan, China [4]Clinical Medicine College, Dali University, Dali, Yunnan, China [5]School of Public Health, Kunming Medical University, Kunming, Yunnan, China [6]Obstetrics Department, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
出处：
DOI： 10.1111/jog.15685
ISSN：

关键词： 1 25(OH)(2)D-3 HTR-8 SVneo cells RNA sequencing UCP3

摘要：
Aim: Vitamin D-3 has been implicated in multiple reproductive events, whereas the effect of its bioactive metabolite 1a, 25 dihydroxyvitamin D3 (1,25(OH) D-2(3)) on transcriptome profile of the placenta is unclear. The aim of this article is to determine transcriptome-wide profile caused by 1,25(OH) D-2(3) in human placental trophoblast cells.Methods: We performed RNA sequencing after stimulation of HTR-8/SVneo cells with 0.1, 1, 10, and 100 nM 1,25(OH)(2)D-3 for 24 h, identified differentially expressed genes by edgeR package (version 3.38.4), and analyzed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways by webtool Metascape. Also, common genes and specific genes in different concentrations of 1,25(OH) D-2(3) were identified.Results: There were 180, 158, 161, and 174 differentially expressed genes after 0.1, 1, 10, and 100 nM 1,25(OH) D-2(3) stimulation, respectively. KEGG pathway analysis displayed that "lipid and atherosclerosis" were significantly enriched at 0.1 and 1 nM 1,25(OH)(2)D-3, while "cytokine-cytokine receptor interaction," "TGF-beta signaling pathway" and "hippo signaling pathway" were significantly enriched in 1, 10, and 100 nM 1,25(OH)(2)D-3. CYP24A1 was a significantly expressed common gene. UCP3 was significantly expressed in low concentrations and might affect energy metabolism. TCF24, EIF3CL, ABCD2, EPHA7, CRLF1, and SECTM1 were specific genes at physiological concentration. Similarly, SPDYE1, IQUB, IL18R1, and ZNF713 were considered as specific genes at supraphysiological concentration.Conclusions: 1,25(OH)(2)D-3 mainly affected the expression of CYP24A1 gene in HTR-8/SVneo cells. Specific genes accounted for the majority of differentially expressed genes at different concentrations. However, their functions need to be further confirmed.

基金：
Digitalization, Development and Application of Biotic Resource [202002AA100007]; Medical Discipline Leader of Yunnan Provincial Health and Family Planning Commission [D-2019016]; National Natural Science Foundation of China [81760273, 81360103, 81860271, 82160281, 82260309]; Yunnan Province "Ten Thousand People Plan" Famous Medical Professional Master Talent [35]; Yunnan Province "Ten Thousand Talents Plan" Youth Top Talent Project [KYQY20210816]; Yunnan Province Medical Discipline Leader Training Object
语种：
外文
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中科院(CAS)分区：
出版当年[2024]版：
无
最新[2023]版：
大类 | 4 区 医学
小类 | 4 区 妇产科学
JCR分区：
出版当年[2023]版：
Q3 OBSTETRICS & GYNECOLOGY
最新[2023]版：
Q3 OBSTETRICS & GYNECOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2023版] 出版当年五年平均 出版前一年[2022版]

第一作者：
第一作者机构： [1]Department of Reproductive Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China [2]Graduate School, Kunming Medical University, Kunming, Yunnan, China
通讯作者：
通讯机构： [1]Department of Reproductive Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China [2]Graduate School, Kunming Medical University, Kunming, Yunnan, China [3]Medical Genetics and Prenatal Diagnosis Center, Kunming Maternal and Child Health Hospital, Kunming, Yunnan, China [4]Clinical Medicine College, Dali University, Dali, Yunnan, China [6]Obstetrics Department, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China [*1]295 Xichang Road, Wuhua District, Kunming, Yunnan, China [*2]No. 2, Danxia Road, Xishan District, Kunming, Yunnan, China
推荐引用方式(GB/T 7714)：
Luo Jiahuan,Tang Li,Zhang Ruopeng,et al.Transcriptome-wide profile of 1a,25 dihydroxyvitamin D-3 in HTR-8/SVneo cells[J].JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH.2023,49(8):2093-2101.doi:10.1111/jog.15685.
APA：
Luo, Jiahuan,Tang, Li,Zhang, Ruopeng,Lv, Mengxin,You, Dingyun&Yuan, Qian.(2023).Transcriptome-wide profile of 1a,25 dihydroxyvitamin D-3 in HTR-8/SVneo cells.JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH,49,(8)
MLA：
Luo, Jiahuan,et al."Transcriptome-wide profile of 1a,25 dihydroxyvitamin D-3 in HTR-8/SVneo cells".JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH 49..8(2023):2093-2101

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