机构：[1]Department of Ultrasound, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, 650118, Yunnan, China[2]Bone and Soft Tissue Tumors Research Centre of Yunnan Province, Department of Orthopaedics, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunming, Yunnan, 650118, China[3]Department of Cancer Center Office, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital, Yunnan Cancer Center), Kunming, Yunnan, 650118, China[4]Department of Thoracic Surgery I&II, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunming, Yunnan, 650118, China[5]Department of Ultrasound, Hohhot First Hospital, Hohhot City, 010059, Inner Mongolia Autonomous Region, China医技科室超声科[6]Ultrasonic Department, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650118, China昆明医科大学附属第一医院[7]Department of Geriatric Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China昆明医科大学附属第一医院老年消化病区干疗科内科科室[8]Molecular Diagnostic Center, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650118, China[9]Department of Endocrinology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650118, China内科科室内分泌科昆明医科大学附属第一医院
The high expression of programmed death 1 (PD-1) is a hallmark of T cell exhaustion, consequently inhibiting the anti-tumor immunity, tumor-associated macrophages (TAMs) aggravate Osteosarcoma (OS) progression. However, PD-1 expression on TAMs in OS metastasis remains unclear. Here, we used scRNA-Seq of 15500 individual cells from human OS lung metastatic lesion, identified thirteen major cell clusters. Our data revealed that tumor-infiltrating lymphocytes (TILs) OS lung metastatic accompanied by accumulation of exhausted T cells and regulatory T cells (Tregs). CD3+ T cells from human OS lung metastatic exhibited lower proliferation than in primary tissue. Importantly, TAMs mainly comprise immunosuppressive M2 phenotype in OS metastasis. Mechanistically, we found that PD-1 of TAMs inhibits the phagocytic potency, further promoting the progression of OS metastasis. Therefore, the study provides a strong technical support for OS immunotherapy based on PD-1 inhibitors.
基金：
National Natural Science Foundation of China [82160125, 81960488]; Joint Special Funds for the Department of Science and Technology of Yunnan Province Kunming Medical University; Science and Technology Innovation Team of Diagnosis and Treatment for Glucolipid Metabolic Diseases in Kunming Medical University [2021J0263]; Yunnan Provincial Department of Education Science Research Fund Project [2023Y0654, 2019EF001 [-236], 2023SYY (BS) 019, 202101AY070001- [171], NCC201925B02]; Start Up Fund for Doctoral Research of Hohhot First Hospital [2023535D14]; Yunnan Fundamental Research Projects [H-2019072]; National Cancer Center Climbing Fund of China [202001AY070001-195, 149,150]; The 535 Talent Project of First Affiliated Hospital of Kunming Medical University [202201AY070001-168,170,136,041,160]; Medical Reserve Talents of Yunnan Province; [CXTD202106]; [2020J0197]; [2022J0235]
第一作者机构：[1]Department of Ultrasound, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, 650118, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[2]Bone and Soft Tissue Tumors Research Centre of Yunnan Province, Department of Orthopaedics, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunming, Yunnan, 650118, China[3]Department of Cancer Center Office, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital, Yunnan Cancer Center), Kunming, Yunnan, 650118, China[*1]Bone and Soft Tissue Tumors Research Centre of Yunnan Province, Department of Orthopaedics, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunming, Yunnan, 650118, China
推荐引用方式(GB/T 7714)：
Zheng Chenhong,Li Heng,Zhao Xiaohui,et al.Expression of PD-1 mitigates phagocytic activities TAM in osteosarcoma[J].HELIYON.2024,10(1):doi:10.1016/j.heliyon.2023.e23498.
APA：
Zheng, Chenhong,Li, Heng,Zhao, Xiaohui,Yang, Siyu,Zhan, Jinqin...&Yao, Zhihong.(2024).Expression of PD-1 mitigates phagocytic activities TAM in osteosarcoma.HELIYON,10,(1)
MLA：
Zheng, Chenhong,et al."Expression of PD-1 mitigates phagocytic activities TAM in osteosarcoma".HELIYON 10..1(2024)
