资源类型：
期刊
WOS体系：
Article
Pubmed体系：
Journal Article
收录情况：
◇ SCIE
文章类型：
论著
机构：
[1]Yan’an Hospital Affiliated To Kunming Medical University, Kunming 650032, China
[2]First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
昆明医科大学附属第一医院
ISSN：
1530-7905
关键词：
Exosome
Pyroptosis
VSMCs
Abdominal aortic aneurysm
LncRNA PVT1
miR-186-5p
摘要：
Abdominal aortic aneurysm (AAA) is a chronic vascular degenerative disease. Vascular smooth muscle cells (VSMCs) are essential for maintaining the integrity of healthy blood vessels. Macrophages play an important role in the inflammatory process of AAA. However, the effect of macrophage-derived exosome LncRNA PVT1 on VSMCs is unclear. Exosomes from M1 macrophages (M1φ-exos) were isolated and identified. The expression of LncRNA PVT1 in M1φ-exos was determined. AAA cell model was constructed by treating VSMCs with Ang-II. AAA cell model was treated with M1φ exosomes transfected with si-LncRNA PVT1 (M1φsi-LncRNA PVT1-exo). VSMCs were transfected with miR-186-5p mimic and oe-HMGB1. Cell viability was detected by CCK-8. The accumulation of LDH was detected by ELISA. Western blot was used to detect the expression of HMGB1, inflammatory factors (IL-6, TNF-α and IL-1β) and pyroptosis-related proteins (GSDMD, N-GSDMD, ASC, NLRP3, Caspase-1 and Cleaved-Capase-1). Cell pyroptosis rate was detected by flow cytometry. At the same time, the targeting relationship between miR-186-5p and LncRNA PVT1 and HMGB1 was verified by double fluorescein experiment. Exosomes from M1φ were successfully extracted. The expression of LncRNA PVT1 in M1φ-exos was significantly increased. M1φ-exo promotes inflammation and pyroptosis of VSMCs. M1φsi-LncRNA PVT1-exos inhibited the inflammation and pyroptosis of VSMCs. LncRNA PVT1 can sponge miR-186-5p mimic to regulate HMGB1 expression. MiR-186-5p mimic further inhibited inflammation and pyroptosis induced by M1φsi-LncRNA PVT1-exos. However, oe-HMGB1 could inhibit the reversal effect of miR-186-5p mimic. LncRNA PVT1 in exosomes secreted by M1φ can regulate HMGB1 by acting as ceRNA on sponge miR-186-5p, thereby promoting cell inflammatory and pyroptosis and accelerating AAA progression.© 2024. The Author(s).
基金：
This work has been supported by the Yunnan Provincial
Science and Technology Plan Project Kun Medical Joint Special
(202201AY070001-184), the Clinical medical technology
(XJ2021003601) and the National Nature Science Foundation of China
(82060294).
被引次数：
5
WOS：
WOS:001176525700001
PubmedID：
38453799
中科院(CAS)分区：
最新[2023]版：
大类
|
3 区
医学
小类
|
3 区
心脏和心血管系统
3 区
毒理学
JCR分区：
最新[2023]版：
Q2
CARDIAC & CARDIOVASCULAR SYSTEMS
Q2
TOXICOLOGY
影响因子：
3.4
最新[2023版]
3
最新五年平均
0
出版当年[2024版]
0
出版当年五年平均
3.4
出版前一年[2023版]
第一作者：
Zhang Jinhui
第一作者机构：
[1]Yan’an Hospital Affiliated To Kunming Medical University, Kunming 650032, China
共同第一作者：
Zhang Xili
通讯作者：
Zhang Jinhui
推荐引用方式(GB/T 7714)：
Zhang Jinhui,Zhang Xili,Liu Xunqiang,et al.M1 Macrophage-Derived Exosome LncRNA PVT1 Promotes Inflammation and Pyroptosis of Vascular Smooth Muscle Cells in Abdominal Aortic Aneurysm by Inhibiting miR-186-5p and Regulating HMGB1[J].CARDIOVASCULAR TOXICOLOGY.2024,24(3):302-320.doi:10.1007/s12012-024-09838-5.
APA：
Zhang Jinhui,Zhang Xili,Liu Xunqiang,Chen Huanjun,Wang Jifeng&Ji Min.(2024).M1 Macrophage-Derived Exosome LncRNA PVT1 Promotes Inflammation and Pyroptosis of Vascular Smooth Muscle Cells in Abdominal Aortic Aneurysm by Inhibiting miR-186-5p and Regulating HMGB1.CARDIOVASCULAR TOXICOLOGY,24,(3)
MLA：
Zhang Jinhui,et al."M1 Macrophage-Derived Exosome LncRNA PVT1 Promotes Inflammation and Pyroptosis of Vascular Smooth Muscle Cells in Abdominal Aortic Aneurysm by Inhibiting miR-186-5p and Regulating HMGB1".CARDIOVASCULAR TOXICOLOGY 24..3(2024):302-320
