Severe drug eruption (SDE), a common skin disease, becomes dangerous when it occurs in patients with human immunodeficiency virus (HIV) infection, however, the molecular mechanisms are poorly understood. Forty patients including HIV+ SDE+ (n = 15), HIV- SDE+ (n = 15) and HIV+ SDE- (n = 10) patients were enrolled in our study. All HIV+ patients were in acquired immune deficiency syndrome (AIDS) stage. Serum cytokines, including TNF-alpha, IFN-gamma, IL-4, IL-13, IL-6, CXCL9, and CCL17, were quantified by ELISA. Epstein-Barr virus (EBV) and cytomegalovirus (CMV) were quantified by RT-qPCR. CD4, CD8, Th1, Th2, TNF-alpha-CD8, and IFN-gamma-CD8 T cell populations were examined by flow cytometry. HIV+ SDE+ patients displayed levels of biochemical indexes that were different from HIV- SDE+ (P < .05). EBV and CMV viral loads were significantly higher in HIV+ SDE+ patients, but not in HIV- SDE+ patients (P < .05). Inflammatory cytokines, such as TNF-alpha and IFN-gamma, were more pronounced in HIV+ SDE+ patients than those in HIV- SDE+ patients (P < .05). Th2/Th1 populations, as well as TNF-alpha secreting and IFN-gamma secreting CD8(+) T cells, were significantly up-regulated in HIV+ SDE+ patients when compared to HIV- SDE+ patients (P < .05). Conversely, the CD4/CD8 ratio was significantly down-regulated in HIV+ SDE+ patients when compared to HIV- SDE+ patients (P < .05). HIV infection confers distinct clinical phenotypes and immune inflammatory mechanisms in SDE. Sustained EBV and CMV activation, unbalanced Th2/Th1 and overactive CD8(+) T cells mediating a pro-inflammatory response could act as distinct mechanisms in the aggravation of SDE in HIV+ SDE+ patients.
基金：
Medical Leadership Foundation of Health and Family Planning Commission of Yunnan Province, China [L-201613]; National Science and Technology Major Project of China [2018ZX10301-101]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81860553, 81560325, 81760741]; Joint Special Fund of Science and Technology Department of Yunnan Province - Kunming Medical University [2017FE467(-005)]; Project for Innovation Team of Department of Science and Technology of Yunnan Province, China [2018HC005]
第一作者机构：[1]Kunming Med Univ, Dept Dermatol & Venerol, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China
通讯作者：
通讯机构：[8]Kunming Med Univ, Affiliated Hosp 1, Kunming 650032, Yunnan, Peoples R China
推荐引用方式(GB/T 7714)：
Tang Jun-Ting,Li Yu-Ye,Zhang Yi,et al.HIV infection confers distinct mechanisms in severe drug eruption: Endogenous virus activation with aberrant Th2/Th1 and CD8(+) T cells function[J].CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY.2020,47(6):1005-1013.doi:10.1111/1440-1681.13266.
APA：
Tang, Jun-Ting,Li, Yu-Ye,Zhang, Yi,Liu, Jun,Guan, Wei...&Kuang, Yi-Qun.(2020).HIV infection confers distinct mechanisms in severe drug eruption: Endogenous virus activation with aberrant Th2/Th1 and CD8(+) T cells function.CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,47,(6)
MLA：
Tang, Jun-Ting,et al."HIV infection confers distinct mechanisms in severe drug eruption: Endogenous virus activation with aberrant Th2/Th1 and CD8(+) T cells function".CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 47..6(2020):1005-1013
