机构:[1]Department of Neurology, First Affiliated Hospital, Kunming Medical University昆明医科大学附属第一医院神经内科[2]Biomedicine Engineering Research Centre, Kunming Medical University[3]Department of Neurology, First Affiliated Hospital, Kunming Medical University, 295 Xi Chang Road, Kunming, Yunnan 650032, P. R. China.[4]Department of Neurology, First Affiliated Hospital, Kunming Medical University, 295 Xi Chang Road, Kunming, Yunnan 650032, P. R. China.
Nuclear-distribution element-like 1 (NDEL1) is associated with the proliferation and migration of neurons. Vascular endothelial growth factor (VEGF) in combination with VEGF receptor-2 (VEGFR-2) regulates the proliferation and migration of neurons. This study was performed to explore undefined alterations in the expression levels of NDEL1 and VEGF/VEGFR-2 within the hippocampus after status epilepticus (SE). Following the creation of pilocarpine-induced epilepsy models using adolescent male C57BL/6 mice, Western blotting and reverse transcription quantitative polymerase chain reaction were applied to assess the levels of NDEL1, VEGF, and VEGFR-2 expression in whole hippocampi at 1, 2, 3, and 4 weeks post-SE, respectively. Immunofluorescent labeling was also employed to detect the colocalization of NDEL1 and VEGF in the hippocampus. Our results indicated that NDEL1 and VEGF have similar patterns of upregulation throughout the hippocampus. Upregulation of VEGFR-2 occurred only in the early stages, and the expression decreased shortly afterward. NDEL1 and VEGF were coexpressed in the cornu ammonis 3 pyramidal cell, granular, and polymorph layers of the dentate gyrus in the hippocampus. This study revealed that NDEL1, VEGF, and VEGFR-2 may work together and are involved in the pathophysiology in the hippocampus after SE.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81601134]; Yunnan Applied Basic Research Projects [2017FE468 (-144)]; Yunnan High-level Talent Project [D-201623]; Program for Science and Technology Innovation Team in Yunnan Province [2018HC008]; China Scholarship CouncilChina Scholarship Council
第一作者机构:[1]Department of Neurology, First Affiliated Hospital, Kunming Medical University
共同第一作者:
通讯作者:
通讯机构:[3]Department of Neurology, First Affiliated Hospital, Kunming Medical University, 295 Xi Chang Road, Kunming, Yunnan 650032, P. R. China.[4]Department of Neurology, First Affiliated Hospital, Kunming Medical University, 295 Xi Chang Road, Kunming, Yunnan 650032, P. R. China.
推荐引用方式(GB/T 7714):
Zhu Lin,Dai Shujuan,Lu Di,et al.Role of NDEL1 and VEGF/VEGFR-2 in Mouse Hippocampus After Status Epilepticus[J].ASN NEURO.2020,12:doi:10.1177/1759091420926836.
APA:
Zhu, Lin,Dai, Shujuan,Lu, Di,Xu, Puying,Chen, Lu...&Wu, Qian.(2020).Role of NDEL1 and VEGF/VEGFR-2 in Mouse Hippocampus After Status Epilepticus.ASN NEURO,12,
MLA:
Zhu, Lin,et al."Role of NDEL1 and VEGF/VEGFR-2 in Mouse Hippocampus After Status Epilepticus".ASN NEURO 12.(2020)